新兴的生物制造方法用于胃肠道类器官以建立患者特异性癌症模型。
Emerging biofabrication approaches for gastrointestinal organoids towards patient specific cancer models.
机构信息
Canary Center at Stanford for Cancer Early Detection, Bio-Acoustic MEMS in Medicine (BAMM) Laboratory, Department of Radiology, School of Medicine Stanford University, Palo Alto, California, 94304-5427, USA.
Canary Center at Stanford for Cancer Early Detection, Bio-Acoustic MEMS in Medicine (BAMM) Laboratory, Department of Radiology, School of Medicine Stanford University, Palo Alto, California, 94304-5427, USA; 3B's Research Group, Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal; ICVS/3B's, PT Government Associate Laboratory, University of Minho, Braga/Guimarães, Portugal.
出版信息
Cancer Lett. 2021 Apr 28;504:116-124. doi: 10.1016/j.canlet.2021.01.023. Epub 2021 Feb 9.
Tissue engineered organoids are simple biomodels that can emulate the structural and functional complexity of specific organs. Here, we review developments in three-dimensional (3D) artificial cell constructs to model gastrointestinal dynamics towards cancer diagnosis. We describe bottom-up approaches to fabricate close-packed cell aggregates, from the use of biochemical and physical cues to guide the self-assembly of organoids, to the use of engineering approaches, including 3D printing/additive manufacturing and external field-driven protocols. Finally, we outline the main challenges and possible risks regarding the potential translation of gastrointestinal organoids from laboratory settings to patient-specific models in clinical applications.
组织工程类器官是一种简单的生物模型，可以模拟特定器官的结构和功能复杂性。在这里，我们回顾了三维（3D）人工细胞构建体在模拟胃肠道动力学以用于癌症诊断方面的发展。我们描述了从使用生化和物理线索来指导类器官的自组装，到使用工程方法，包括 3D 打印/增材制造和外部场驱动协议，来制造紧密堆积的细胞聚集体的自下而上的方法。最后，我们概述了将胃肠道类器官从实验室环境转化为临床应用中患者特异性模型的主要挑战和潜在风险。
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