Soyal A, Gokmen T G, Kayar B, Koksal F
Department of Medical Microbiology, Medical Faculty, Cukurova University, 01330, Adana, Turkey.
Department of Microbiology, Ceyhan Veterinary Faculty, Cukurova University, 01330, Adana, Turkey.
Trop Biomed. 2016 Jun 1;33(2):260-267.
Leishmaniasis is a multisystem disease, and have a broad spectrum ranging from skin lesions to systemic disease. Therefore diagnosis must be supported with laboratory results. We analyzed 153 smears, aspiration, blood and bone narrow samples collected from patients suspected with cutaneous and visceral leishmaniasis. The specificity and sensitivity of the four methods (culture, smear, miniexon-PCR-RFLP and ITS1-PCR-RFLP) were detected and Leishmania species were determined. The ITS1-PCR-RFLP method was found that the highest sensitivity and specifity. L. infantum and L. tropica were identified by molecular methods from samples. As a result, ITS-1-PCR has a high sensitivity and specificity and easily applicable method. However, it requires the miniexon-PCR or ITS1 sequencing the discrimination of the L. donovani complex. L. infantum is a agent both visceral and cutaneous leishmaniasis in our region.
利什曼病是一种多系统疾病，其范围广泛，从皮肤病变到全身性疾病。因此，诊断必须有实验室结果支持。我们分析了从疑似皮肤利什曼病和内脏利什曼病患者身上采集的153份涂片、抽吸物、血液和骨髓样本。检测了四种方法（培养、涂片、小外显子-PCR-RFLP和ITS1-PCR-RFLP）的特异性和敏感性，并确定了利什曼原虫种类。发现ITS1-PCR-RFLP方法具有最高的敏感性和特异性。通过分子方法从样本中鉴定出婴儿利什曼原虫和热带利什曼原虫。结果表明，ITS-1-PCR具有高敏感性和特异性，且是易于应用的方法。然而，它需要小外显子-PCR或ITS1测序来鉴别杜氏利什曼原虫复合体。婴儿利什曼原虫是我们地区内脏利什曼病和皮肤利什曼病的病原体。
