Li Zhongqiu, Zeng Wenbo, Yang Yufeng, Zhang Peijun, Zhou Zhengbing, Li Yuanyuan, Guo Yunhai, Zhang Yi
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai 200025, China.
Rizhang Center for Disease Control and Prevention, Rizhao 276803, China.
Trop Med Infect Dis. 2022 Aug 10;7(8):176. doi: 10.3390/tropicalmed7080176.
Leishmaniasis is a neglected tropical disease that seriously influences global public health. Among all the parasitic diseases, leishmaniasis is the third most common cause of morbidity after malaria and schistosomiasis. Circular RNAs (circRNAs) are a new type of noncoding RNAs that are involved in the regulation of biological and developmental processes. However, there is no published research on the function of circRNAs in leishmaniasis. This is the first study to explore the expression profiles of circRNAs in leishmaniasis. GO and KEGG analyses were performed to determine the potential function of the host genes of differentially expressed circRNAs. CircRNA-miRNA-mRNA (ceRNA) regulatory network analysis and protein-protein interaction (PPI) networks were analyzed by R software and the STRING database, respectively. A total of 4664 significant differentially expressed circRNAs were identified and compared to those in control groups; a total of 1931 were up-regulated and 2733 were down-regulated. The host genes of differentially expressed circRNAs were enriched in ubiquitin-mediated proteolysis, endocytosis, the MAPK signaling pathway, renal cell carcinoma, autophagy and the ErbB signaling pathway. Then, five hub genes (, , , , and ) were identified. This study provides new evidence of the change of differentially expressed circRNAs and its potential function in leishmaniasis. These results may provide novel insights and evidence for the diagnosis and treatment of leishmaniasis.
利什曼病是一种被忽视的热带疾病，严重影响全球公共卫生。在所有寄生虫病中，利什曼病是仅次于疟疾和血吸虫病的第三大常见发病原因。环状RNA（circRNAs）是一类新型的非编码RNA，参与生物和发育过程的调控。然而，关于circRNAs在利什曼病中的功能尚无已发表的研究。这是首次探索利什曼病中circRNAs表达谱的研究。进行了基因本体（GO）和京都基因与基因组百科全书（KEGG）分析，以确定差异表达circRNAs宿主基因的潜在功能。分别通过R软件和STRING数据库分析了circRNA- miRNA- mRNA（ceRNA）调控网络和蛋白质-蛋白质相互作用（PPI）网络。共鉴定出4664个显著差异表达的circRNAs，并与对照组进行比较；其中1931个上调，2733个下调。差异表达circRNAs的宿主基因富集于泛素介导的蛋白水解、内吞作用、丝裂原活化蛋白激酶（MAPK）信号通路、肾细胞癌、自噬和表皮生长因子受体（ErbB）信号通路。然后，鉴定出五个枢纽基因（、、、和）。本研究为利什曼病中差异表达circRNAs的变化及其潜在功能提供了新的证据。这些结果可能为利什曼病的诊断和治疗提供新的见解和证据。
