日本栝楼主要成分天花酸对人血小板聚集及花生四烯酸代谢的体外作用

In vitro effect of trichosanic acid, a major component of Trichosanthes japonica on platelet aggregation and arachidonic acid metabolism in human platelets.

作者信息

Takenaga M, Hirai A, Terano T, Tamura Y, Kitagawa H, Yoshida S

机构信息

Faculty of Pharmaceutical Sciences, Chiba University, Japan.

出版信息

Prostaglandins Leukot Essent Fatty Acids. 1988 Feb;31(2):65-72. doi: 10.1016/0952-3278(88)90078-6.

DOI:10.1016/0952-3278(88)90078-6

PMID:3357911

Abstract

The in vitro effect of trichosanic acid (TCA; C18:3, omega-5), a major component of Trichosanthes japonica, on platelet aggregation and arachidonic acid (AA) metabolism in human platelets was studied. TCA dose-dependently suppressed platelet aggregation of platelet rich plasma and washed platelets. TCA decreased collagen (50 micrograms/ml)-stimulated production of thromboxane B2 (TXB2) and 12-hydroxyhepta-decatrienoic acid (HHT) in a dose-dependent manner, while that of 12-hydroxyeicosatetraenoic acid (12-HETE) was rather enhanced. The conversion of exogenously added [14C]AA to [14C]TXB2 and [14C]HHT in washed platelets was dose-dependently reduced by the addition of TCA, while that to [14C]12-HETE was increased. Similar observations were obtained when linolenic acid (LNA; C18:3, omega-3) was used. These results suggest that TCA may decrease TXA2 formation in platelets, probably due to the inhibition of cyclooxygenase pathway, and thereby reduce platelet aggregation.

摘要

研究了日本栝楼的主要成分天花酸（TCA；C18:3，ω-5）对人血小板聚集和花生四烯酸（AA）代谢的体外作用。TCA剂量依赖性地抑制富血小板血浆和洗涤血小板的血小板聚集。TCA以剂量依赖性方式降低胶原蛋白（50微克/毫升）刺激的血栓素B2（TXB2）和12-羟基十七碳三烯酸（HHT）的产生，而12-羟基二十碳四烯酸（12-HETE）的产生则略有增强。在洗涤血小板中，添加TCA可剂量依赖性地减少外源性添加的[14C]AA向[14C]TXB2和[14C]HHT的转化，而向[14C]12-HETE的转化则增加。使用亚麻酸（LNA；C18:3，ω-3）时也得到了类似的结果。这些结果表明，TCA可能通过抑制环氧化酶途径减少血小板中TXA2的形成，从而降低血小板聚集。