Takahashi Chisato, Yamada Tomomi, Yagi Shinya, Murai Takaaki, Muto Shunsuke
Magnetic Powder Metallurgy Research Center, National Institute of Advanced Industrial Science and Technology, 2266-98, Anagahora, Shimoshidami, Moriyama-ku, Nagoya, Aichi 463-8560, Japan; Laboratoire Matériaux et Phénomènes Quantiques, Université de Paris, Bâtiment Condorcet, 10 rue Alice Domon et Léonie Duquet, Case courrier 7021, 75205 Paris CEDEX 13, France.
Pharmaceutical Engineering, School of Pharmacy, Aichi Gakuin University, 1-100, Kusumoto-cho, Chikusa-ku, Nagoya, Aichi 464-8650, Japan.
Mater Sci Eng C Mater Biol Appl. 2021 Feb;121:111718. doi: 10.1016/j.msec.2020.111718. Epub 2020 Dec 15.
Biofilm infections present a serious problem because antibacterial drugs are not effective against mature biofilms or biofilms formed by drug-resistant bacteria. To address this issue, we developed a drug delivery system based on metal-decorated polymeric particles. Polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer (Soluplus®) is an amphiphilic polymer used in biomedical formulations, while silver nanoparticles are widely acknowledged to have high antibacterial activity. We prepared silver-decorated Soluplus® micelle nanoparticles with high antibacterial activity using the emulsion solvent diffusion method. Decoration of Soluplus® micelles with silver nanoparticles was found to increase their antibacterial activity. Scanning transmission electron microscopy-cathodoluminescence (STEM-CL) spectroscopy allows imaging of the spatial distribution of labeled targets and the chemical identification of materials. However, STEM-CL spectroscopy of fragile polymer materials is challenging. We optimized the STEM-CL spectroscopy technique to determine the distribution of silver nanoparticles in Soluplus® micelles. Additionally, the surface plasmon properties of the silver nanoparticles were successfully characterized without deactivation. The developed silver-decorated Soluplus® nanoparticles were effective against biofilm infections and have the potential to be applied for other biofilm-related diseases. Additionally, the optimized STEM-CL spectroscopy technique is expected to contribute to the analysis and imaging of fragile polymer materials, as well as other soft materials such as cells and tissues.
生物膜感染是一个严重的问题，因为抗菌药物对成熟生物膜或由耐药细菌形成的生物膜无效。为了解决这个问题，我们开发了一种基于金属修饰聚合物颗粒的药物递送系统。聚乙烯己内酰胺-聚醋酸乙烯酯-聚乙二醇接枝共聚物（Soluplus®）是一种用于生物医学制剂的两亲性聚合物，而银纳米颗粒被广泛认为具有高抗菌活性。我们使用乳液溶剂扩散法制备了具有高抗菌活性的银修饰Soluplus®胶束纳米颗粒。发现用银纳米颗粒修饰Soluplus®胶束可提高其抗菌活性。扫描透射电子显微镜-阴极发光（STEM-CL）光谱法可以对标记靶标的空间分布进行成像，并对材料进行化学鉴定。然而，对易碎聚合物材料进行STEM-CL光谱分析具有挑战性。我们优化了STEM-CL光谱技术，以确定银纳米颗粒在Soluplus®胶束中的分布。此外，成功表征了银纳米颗粒的表面等离子体特性，且未使其失活。所开发的银修饰Soluplus®纳米颗粒对生物膜感染有效，并有潜力应用于其他与生物膜相关的疾病。此外，优化后的STEM-CL光谱技术有望有助于对易碎聚合物材料以及其他软材料（如细胞和组织）进行分析和成像。
