Key Laboratory of Organ Development and Regeneration of Zhejiang Province, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311100 Zhejiang, China.
College of Life Sciences, Zhejiang University, Hangzhou 310013 Zhejiang, China.
Biol Open. 2021 Mar 8;10(3):bio056382. doi: 10.1242/bio.056382.
LIM homeobox 9 (Lhx9) is a member of the LIM homeodomain transcription factor family, which expresses and functions in various vertebrate tissues, such as the gonads and pineal gland. Previous studies on in zebrafish have mainly focused on the brain. However, little is known about the expression pattern of during embryogenesis. Here, we detected expression in zebrafish embryos using whole-mount hybridization and found expressed in heart, pectoral fin, and retina during their development in zebrafish. We then detailed the expression of in retinal development. To further investigate the function of Lhx9 in retinogenesis, we performed morpholino (MO) knockdown experiments and found that upon knockdown by MO, larvae presented normal eye development, retinal neural development, differentiation, proliferation, apoptosis, and responses to light stimulus. We not only elaborated the expression pattern of in zebrafish embryogenesis, but we also demonstrated that knockdown by morpholino does not affect the zebrafish retinal development, and our study provides data for further understanding of the role of Lhx9 in zebrafish retinal development.
LIM 同源盒蛋白 9（Lhx9）是 LIM 同源结构域转录因子家族的成员，在各种脊椎动物组织中表达和发挥作用，如性腺和松果体。先前对斑马鱼中的 的研究主要集中在大脑上。然而，关于 在胚胎发生过程中的表达模式知之甚少。在这里，我们使用全胚胎原位杂交检测了斑马鱼胚胎中的 表达，发现 在心脏、胸鳍和视网膜发育过程中表达。然后，我们详细描述了 在视网膜发育中的表达。为了进一步研究 Lhx9 在视网膜发生中的功能，我们进行了 morpholino（MO）敲低实验，发现 MO 敲低后，幼虫的眼睛发育、视网膜神经发育、分化、增殖、凋亡和对光刺激的反应均正常。我们不仅详细阐述了 在斑马鱼胚胎发生中的表达模式，而且还证明 morpholino 敲低 不会影响斑马鱼视网膜的发育，我们的研究为进一步了解 Lhx9 在斑马鱼视网膜发育中的作用提供了数据。
