S 变异株 SARS-CoV-2 谱系 B1.1.7 与 TaqPath 聚合酶链反应检测样本中的病毒载量显著升高相关。
S-Variant SARS-CoV-2 Lineage B1.1.7 Is Associated With Significantly Higher Viral Load in Samples Tested by TaqPath Polymerase Chain Reaction.
机构信息
Public Health England, Birmingham, United Kingdom.
University Hospitals Birmingham NHS Foundation Trust, Birmingham, United Kingdom.
出版信息
J Infect Dis. 2021 May 28;223(10):1666-1670. doi: 10.1093/infdis/jiab082.
Abstract
A SARS-CoV-2 variant B1.1.7 containing mutation Δ69/70 has spread rapidly in the United Kingdom and shows an identifiable profile in ThermoFisher TaqPath RT-qPCR, S gene target failure (SGTF). We analyzed recent test data for trends and significance. Linked cycle threshold (Ct) values for respiratory samples showed that a low Ct for ORF1ab and N were clearly associated with SGTF. Significantly more SGTF samples had higher inferred viral loads between 1×107 and 1×108. Our conclusion is that patients whose samples exhibit the SGTF profile are more likely to have high viral loads, which may explain higher infectivity and rapidity of spread.
摘要
一种含有突变 Δ69/70 的 SARS-CoV-2 变体 B1.1.7 在英国迅速传播，并在 ThermoFisher TaqPath RT-qPCR 中显示出可识别的 S 基因靶标失败（SGTF）特征。我们分析了最近的测试数据以了解趋势和意义。与呼吸道样本相关联的循环阈值（Ct）值表明，ORF1ab 和 N 的低 Ct 与 SGTF 明显相关。具有更高推断病毒载量（1×107 到 1×108 之间）的 SGTF 样本数量明显更多。我们的结论是，样本显示 SGTF 特征的患者更有可能具有高病毒载量，这可能解释了更高的传染性和快速传播性。
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