Bharda Anahita Vispi, Jung Hyun Suk
Division of Chemistry and Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon-si, Gangwon-do, 24341, South Korea.
Appl Microsc. 2019 Oct 25;49(1):9. doi: 10.1186/s42649-019-0011-7.
Contemporary microscopic imaging at near-atomic resolution of diverse embodiments in liquid environment has gained keen interest. In particular, Electron Microscopy (EM) can provide comprehensive framework on the structural and functional characterization of samples in liquid phase. In the past few decades, liquid based electron microscopic modalities have developed tremendously to provide insights into various backgrounds like biological, chemical, nanoparticle and material researches. It serves to be a promising analytical tool in deciphering unique insights from solvated systems. Here, the basics of liquid electron microscopy with few examples of its applications are summarized in brief. The technical developments made so far and its preference over other approaches is shortly presented. Finally, the experimental limitations and an outlook on the future technical advancement for liquid EM have been discussed.
在液体环境中以近原子分辨率对各种样本进行当代微观成像已引起了广泛关注。特别是，电子显微镜（EM）可以为液相样本的结构和功能表征提供全面的框架。在过去几十年中，基于液体的电子显微镜技术有了巨大发展，能够为生物学、化学、纳米颗粒和材料研究等各种领域提供深入见解。它是一种很有前景的分析工具，可用于解读溶剂化体系中的独特信息。在此，简要总结了液体电子显微镜的基本原理及其一些应用实例。还简要介绍了迄今为止所取得的技术进展以及它相对于其他方法的优势。最后，讨论了实验局限性以及液体电子显微镜未来技术进步的展望。
