序列糖基化苷元 A 产生新型三萜类双糖皂苷。
Production of a new triterpenoid disaccharide saponin from sequential glycosylation of ganoderic acid A by 2 Bacillus glycosyltransferases.
机构信息
Department of Biological Sciences and Technology, National University of Tainan, Tainan, Taiwan.
Department of Pharmacy, Chia Nan University of Pharmacy and Science, Tainan, Taiwan.
出版信息
Biosci Biotechnol Biochem. 2021 Feb 24;85(3):687-690. doi: 10.1093/bbb/zbaa055.
Ganoderic acid A (GAA) is a lanostane-type triterpenoid, isolated from medicinal fungus Ganoderma lucidum, and possesses multiple bioactivities. In the present study, GAA was sequentially biotransformed by 2 recently discovered Bacillus glycosyltransferases (GT), BtGT_16345 and BsGT110, and the final product was purified and identified as a new compound, GAA-15,26-O-β-diglucoside, which showed 1024-fold aqueous solubility than GAA.
灵芝酸 A（GAA）是一种从药用真菌灵芝中分离得到的羊毛甾烷型三萜，具有多种生物活性。在本研究中，GAA 先后被两种最近发现的芽孢杆菌糖苷转移酶（GT），BtGT_16345 和 BsGT110 生物转化，最终产物被纯化并鉴定为一种新化合物，GAA-15,26-O-β-二葡萄糖苷，其在水中的溶解度比 GAA 高 1024 倍。
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