Glycosylation of Ganoderic Acid G by Glycosyltransferases.

is a medicinal fungus abundant in triterpenoids, its primary bioactive components. Although numerous triterpenoids have already been identified, rare triterpenoid saponins were recently discovered. To create novel saponins, ganoderic acid G (GAG) was selected for biotransformation using four glycosyltransferases (GTs) including BtGT_16345 from the GA A07 strain and three GTs (BsGT110, BsUGT398, and BsUGT489) from the ATCC 6633 strain. The results showed that BsUGT489 catalyzed the glycosylation of GAG to GAG-3---glucoside, while BsGT110 catalyzed the glycosylation of GAG to GAG-26---glucoside, which showed 54-fold and 97-fold greater aqueous solubility than that of GAG, respectively. To our knowledge, these two GAG saponins are new compounds. The glycosylation specificity of the four GTs highlights the possibility of novel triterpenoid saponin production in the future.