采用聚乙二醇沉淀法对血清中甲状腺球蛋白自身抗体进行灵敏检测。

Sensitive assay for thyroglobulin autoantibodies in serum employing polyethylene glycol for precipitation.

作者信息

Laurberg P, Pedersen K M

机构信息

Second University Clinic of Internal Medicine, Aarhus Kommunehospital, Denmark.

出版信息

Scand J Clin Lab Invest. 1988 Apr;48(2):137-40. doi: 10.3109/00365518809085405.

DOI:10.3109/00365518809085405

PMID:3358095

Abstract

A sensitive assay for thyroglobulin autoantibodies (Tg-ab) is described. The assay is based on prolonged incubation of [125I]Tg with serum followed by precipitation of antibody bound [125I]Tg by polyethylene glycol (PEG). The PEG concentration was chosen to give a low precipitation of free [125I]Tg while effectively precipitating Tg-ab-bound [125I]Tg. The lowest reference employed contained 19.5 MRC U/l Tg-ab. This was always different from an incubate without Tg-ab. Tg standard added to samples interfered in the assay giving spuriously low Tg-ab values as in other Tg-ab assays. Tg-ab added to samples was recovered quantitatively. The interassay coefficient of variation was 19.8% and the intra-assay coefficient of variation 11.3%. Only eight of 66 serum samples with various amounts of Tg-ab, measured with the new assay, had levels of Tg-ab detectable by the passive haemagglutination test.

摘要

本文描述了一种检测甲状腺球蛋白自身抗体（Tg-ab）的灵敏方法。该方法基于将[125I]Tg与血清长时间孵育，然后用聚乙二醇（PEG）沉淀与抗体结合的[125I]Tg。选择PEG浓度是为了使游离[125I]Tg的沉淀率较低，同时有效地沉淀与Tg-ab结合的[125I]Tg。所采用的最低参考值含有19.5 MRC U/l Tg-ab。这总是与不含Tg-ab的孵育物不同。向样品中添加Tg标准品会干扰检测，导致Tg-ab值出现假性降低，其他Tg-ab检测方法也存在这种情况。向样品中添加的Tg-ab可被定量回收。批间变异系数为19.8%，批内变异系数为11.3%。用新方法检测的66份含有不同量Tg-ab的血清样本中，只有8份的Tg-ab水平可通过被动血凝试验检测到。