Miyoshi M, Hirakawa S, Hasegawa K, Mitsunaga M, Takehisa Y
Nihon Naibunpi Gakkai Zasshi. 1977 Nov 20;53(11):1268-78. doi: 10.1507/endocrine1927.53.11_1268.
It is well known that the antithyroglobulin (anti-Tg) antibody plays an important role in the pathogenesis of autoimmune thyroiditis (chronic thyroiditis). The anti-Tg antibody is detected by double diffusion in agar gel, the fluorescent antibody technique and the tanned red cell haemagglutination test (TRC). The most widely used method is the TRC test, but it is negative in about 30 percent of the patients with chronic thyroiditis. In this paper, we have reported the detection of the anti-Tg antibody in serum using a modified Farr's method. In our method, PEG was used instead of ammonium sulfate to precipitate the immune complex formed in vitro between labelled Tg and the autoantibody. Percent 125I-Tg precipitated was 4.7 +/- 3.1 percent in normal controls; 20.4 +/- 11.4 percent in TRC negative sera were detected by this method. A good correlation was found between TRC titer and percent 125I-Tg precipitated by the PEG method in patients with chronic thyroiditis. By this method, the anti-Tg antibody was also detected in the sera of rabbits immunised with human Tg earlier than that detected by the TRC and double diffusion tests. The sensitivity and simplicity of this method provide a useful tool in detecting the anti-Tg antibody in clinical as well as in experimental work.
众所周知，抗甲状腺球蛋白（anti-Tg）抗体在自身免疫性甲状腺炎（慢性甲状腺炎）的发病机制中起重要作用。抗Tg抗体可通过琼脂凝胶双向扩散、荧光抗体技术及鞣酸红细胞血凝试验（TRC）检测。应用最广泛的方法是TRC试验，但约30%的慢性甲状腺炎患者该试验呈阴性。本文报道了用改良的Farr法检测血清中的抗Tg抗体。在我们的方法中，用聚乙二醇（PEG）代替硫酸铵沉淀标记的Tg与自身抗体在体外形成的免疫复合物。正常对照中125I-Tg沉淀百分比为4.7±3.1%；用该方法在TRC阴性血清中检测到20.4±11.4%。在慢性甲状腺炎患者中，TRC滴度与PEG法沉淀的125I-Tg百分比之间存在良好的相关性。用该方法，在用人类Tg免疫的兔血清中检测到抗Tg抗体也早于TRC和双向扩散试验。该方法的敏感性和简便性为临床及实验工作中检测抗Tg抗体提供了一种有用的工具。
