Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic.
Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK.
Methods Mol Biol. 2020;2168:273-297. doi: 10.1007/978-1-0716-0724-4_13.
Single-molecule techniques provide insights into the heterogeneity and dynamics of ensembles and enable the extraction of mechanistic information that is complementary to high-resolution structural techniques. Here, we describe the application of single-molecule Förster resonance energy transfer to study the dynamics of integral membrane protein complexes on timescales spanning sub-milliseconds to minutes (10-10 s).
单分子技术提供了对集合体异质性和动态的深入了解，并能够提取与高分辨率结构技术互补的机制信息。在这里，我们描述了单分子Förster 共振能量转移在亚毫秒到分钟（10-10 s）时间尺度上研究整体膜蛋白复合物动力学的应用。
