通过单分子荧光在多个时间尺度上监测膜蛋白的动力学。
Dynamics of Membrane Proteins Monitored by Single-Molecule Fluorescence Across Multiple Timescales.
机构信息
Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic.
Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK.
出版信息
Methods Mol Biol. 2020;2168:273-297. doi: 10.1007/978-1-0716-0724-4_13.
Single-molecule techniques provide insights into the heterogeneity and dynamics of ensembles and enable the extraction of mechanistic information that is complementary to high-resolution structural techniques. Here, we describe the application of single-molecule Förster resonance energy transfer to study the dynamics of integral membrane protein complexes on timescales spanning sub-milliseconds to minutes (10-10 s).
单分子技术提供了对集合体异质性和动态的深入了解，并能够提取与高分辨率结构技术互补的机制信息。在这里，我们描述了单分子Förster 共振能量转移在亚毫秒到分钟（10-10 s）时间尺度上研究整体膜蛋白复合物动力学的应用。
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