Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden.
Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden.
Curr Opin Struct Biol. 2020 Dec;65:61-68. doi: 10.1016/j.sbi.2020.05.006. Epub 2020 Jul 4.
Single-molecule fluorescence microscopy has long been appreciated as a powerful tool to study the structural dynamics that enable biological function of macromolecules. Recent years have witnessed the development of more complex single-molecule fluorescence techniques as well as powerful combinations with structural approaches to obtain mechanistic insights into the workings of various molecular machines and protein complexes. In this review, we highlight these developments that together bring us one step closer to a dynamic understanding of biological processes in atomic details.
单分子荧光显微镜长期以来一直被认为是研究大分子实现生物功能的结构动力学的有力工具。近年来，人们开发出了更复杂的单分子荧光技术，并与结构方法进行了有力的结合，从而深入了解了各种分子机器和蛋白质复合物的工作机制。在这篇综述中，我们强调了这些进展，它们共同使我们更接近于从原子细节动态理解生物过程。
