Faculty of Pharmacy, Pharmaceutical Technology Department, Bezmialem Vakif University, Istanbul, Turkey.
Hamidiye School of Medicine, Department of Medical Biochemistry, University of Health Sciences, Istanbul, Turkey.
Pharm Dev Technol. 2021 Apr;26(4):444-454. doi: 10.1080/10837450.2021.1887892. Epub 2021 Feb 18.
Conventional formulations can not achieve wound healing efficiently and fail to accelerate wound regeneration. To overcome these problems, it was planned to develop nanoformulations that perform a positive effect on the wound healing duration and are suitable for topical use. In this study, liposomal film formulations that encapsulated d-panthenyl triacetate (PTA) and coenzyme Q10 (CoQ10) were optimized by using response surface methodology (RSM) and were analyzed for their wound healing efficacy and cytotoxicity on fibroblast (CCD1079 Sk) and keratinocyte (HEKa) cells. Swelling index, puncture strength, and puncture deformation values, which were choosen as dependent variables for the liposomal film formulation were found as 556.9% ± 21.3, 3.98 ± 0.98 N/mm, and 6.57% ± 1.12, respectively. Cumulative release of 65.32% for PTA and 12.23% for CoQ10 was obtained after 24 hours of release study in sink conditions. The cytotoxicity and wound healing assay results suggested that optimum formulation could be used safely on fibroblast and keratinocyte cells and provided wound closure entirely after 24 h. Consequently, the optimum liposomal film containing PTA and CoQ10 formulations could be proposed as an innovative approach in wound healing treatment, considering their release, mechanical properties, stability, and effectiveness.
常规制剂不能有效地实现伤口愈合，也不能加速伤口再生。为了克服这些问题，计划开发纳米制剂，对伤口愈合时间产生积极影响，并适合局部使用。在这项研究中，通过响应面法（RSM）优化了包裹二泛醇三乙酸酯（PTA）和辅酶 Q10（CoQ10）的脂质体薄膜制剂，并分析了其对成纤维细胞（CCD1079 Sk）和角质形成细胞（HEKa）的伤口愈合功效和细胞毒性。选择作为脂质体薄膜制剂的依赖变量的溶胀指数、穿刺强度和穿刺变形值分别为 556.9%±21.3、3.98±0.98 N/mm 和 6.57%±1.12。在 24 小时的释放研究中，在溶出条件下，PTA 的累积释放率为 65.32%，CoQ10 的累积释放率为 12.23%。细胞毒性和伤口愈合试验结果表明，最佳配方可安全用于成纤维细胞和角质形成细胞，在 24 小时后可完全闭合伤口。因此，考虑到其释放、机械性能、稳定性和有效性，含有 PTA 和 CoQ10 的最佳脂质体薄膜制剂可以作为一种创新的伤口愈合治疗方法。
