Faculty of Basic Medical Science, Yunnan University of Chinese Medicine, Kunming, China.
Pak J Pharm Sci. 2020 Jul;33(4):1493-1501.
To investigate the potential roles of the traditional Chinese medicine Yupingfengsan and Siwutang compound formula (YS) in chronic obstructive pulmonary disease (COPD) rats, wistar rats were assigned to control, YS-treated and COPD model groups. The COPD rats model were established by passive smoking and intratracheal instillation of lipopolysaccharide (LPS). Histological changes were detected by hematoxylin/eosin (HE) staining. Protein levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, transforming growth factor (TGF)-β1 and phosphorylated-smad2 (p-smad2) were determined by western blot assay. The activities of super oxide dismutase (SOD), glutathion peroxidase (GSH-Px) and the content of malondialdehyde (MDA) in the serum were estimated by biochemical methods. Relative mRNA levels of TNF-α, IL-6 and TGF-β1 were measured by quantitative real-time polymerase chain reaction (RT-PCR) analysis. The results showed that YS enhanced the above oxidase activity and decreased the yield of MDA, and reduced the levels of TNF-α, IL-6, TGF-β1 and p-smad2 in YS-treated COPD rats compared with the COPD rats. Our results suggested that YS produced the beneficial effects in COPD rats by antiinflammatory and antioxidative actions. Moreover, our research indicated that YS produced antiinflammatory effects in COPD rats by inhibiting the expression of inflammatory cytokines, possibly through suppressing the TGF-β1/Smad2 signaling pathway.
为了研究中药玉屏风散和四逆汤复方（YS）在慢性阻塞性肺疾病（COPD）大鼠中的潜在作用，将 Wistar 大鼠分为对照组、YS 治疗组和 COPD 模型组。通过被动吸烟和气管内滴注脂多糖（LPS）建立 COPD 大鼠模型。通过苏木精/伊红（HE）染色检测组织学变化。通过 Western blot 检测肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6、转化生长因子（TGF）-β1 和磷酸化-smad2（p-smad2）的蛋白水平。通过生化方法测定血清中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）的活性和丙二醛（MDA）的含量。通过定量实时聚合酶链反应（RT-PCR）分析测定 TNF-α、IL-6 和 TGF-β1 的相对 mRNA 水平。结果表明，YS 增强了上述氧化酶的活性，降低了 MDA 的产量，并降低了 COPD 大鼠中 TNF-α、IL-6、TGF-β1 和 p-smad2 的水平，与 COPD 大鼠相比。我们的研究结果表明，YS 通过抗炎和抗氧化作用对 COPD 大鼠产生有益影响。此外，我们的研究表明，YS 通过抑制炎症细胞因子的表达对 COPD 大鼠产生抗炎作用，可能通过抑制 TGF-β1/Smad2 信号通路。
