Department of Clinical Medicine, School of Medicine, Zhejiang University City College, Hangzhou, Zhejiang, PR China.
Department of Basic Medicine, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, PR China.
Pak J Pharm Sci. 2020 Jul;33(4):1527-1533.
To investigate the effects of apigenin on the injury caused by oxygen and glucose deprivation in neurons and the underlying mechanisms, primary cultured rat hippocampal neurons were incubated with apigenin for 90 min before a 2-h oxygen and glucose deprivation followed by a 24-h reperfusion (OGD/R). Subsequently, cell viability, lactate dehydrogenase (LDH) leakage rate, apoptotic rate of neurons and activity of the sodium pump were assessed. In addition, activity of the sodium pump was also examined in the hippocampus of SD rats injected intraperitoneally with apigenin 90 min before a 10-min global cerebral ischemia/24-h reperfusion. The results showed that cell viability and activity of the sodium pump markedly decreased but LDH leakage rate and apoptotic rate significantly increased in OGD/R-treated neurons. However, pretreatment with apigenin (20-50μmol/L) reversed the changes dose-dependently. Compared to sham controls, activity of the sodium pump was significantly suppressed in global ischemia/reperfusion rats; application of apigenin (200mg/kg) restored the activity of the sodium pump. Furthermore, the neuroprotective effect of apigenin was blocked partly by the sodium pump inhibitor ouabain. Our findings provide the evidence that apigenin has a neuroprotective effect against OGD/R injury and the protective effect may be associated with its ability to improve sodium pump activity.
为了研究芹菜素对神经元缺氧缺糖损伤的影响及其作用机制，原代培养的大鼠海马神经元在缺氧缺糖（OGD/R）前 90 分钟用芹菜素孵育，然后进行 2 小时的 OGD/R 及 24 小时的再灌注。随后，评估细胞活力、乳酸脱氢酶（LDH）漏出率、神经元凋亡率和钠泵活性。此外，在 SD 大鼠腹腔注射芹菜素 90 分钟后进行 10 分钟全脑缺血/24 小时再灌注，还检测了海马中的钠泵活性。结果表明，OGD/R 处理的神经元中细胞活力和钠泵活性显著降低，但 LDH 漏出率和凋亡率显著增加。然而，用芹菜素（20-50μmol/L）预处理可呈剂量依赖性逆转这些变化。与假手术对照组相比，全脑缺血/再灌注大鼠的钠泵活性明显受到抑制；应用芹菜素（200mg/kg）可恢复钠泵的活性。此外，钠泵抑制剂哇巴因部分阻断了芹菜素的神经保护作用。我们的研究结果提供了证据表明，芹菜素对 OGD/R 损伤具有神经保护作用，这种保护作用可能与其提高钠泵活性的能力有关。
