Department of Chemistry, Faculty of Mathematics and Natural Sciences,Universitas Halu Oleo, Kendari, Southeast Sulawesi, Indonesia.
Pak J Pharm Sci. 2020 Jul;33(4):1631-1639.
Isolation and identification of secondary metabolite from n-hexane fraction of lichen Usnea longissima Ach. and its bioactivity as antibacterial have been carried out. The isolation of chemical compounds was performed by using Gravity Column Chromatography (GCC), solvent systems as mobile phase (n-hexane, ethyl acetate), and Thin Layer Chromatography (TLC). The form of isolated compound was yellow needle crystals. The result of 1D-NMR (H and C-NMR) data showed that the isolated compound was usnic acid. Antibacterial bioactivity testing was conducted by using the paper disk diffusion method indicated that the usnic acid compound was actively inhibited the growth of E. coli (ATCC35218) and S. aureus (ATCC25923) at the concentrations of 500mg/mL and 1000mg/mL with inhibition zone between 12mm and 17mm. In addition, S. typhi (YCTC) was only inhibited at the concentration of 1000mg/mL with inhibition zone of 14mm.
已对长松萝（Usnea longissima Ach.）正己烷级分中的次生代谢产物进行了分离和鉴定，并对其作为抗菌剂的生物活性进行了研究。通过使用重力柱层析（GCC），溶剂系统（正己烷，乙酸乙酯）和薄层色谱（TLC）作为流动相，对化合物进行了分离。分离得到的化合物呈黄色针状晶体。1D-NMR（H 和 C-NMR）数据分析结果表明，分离得到的化合物为松萝酸。通过纸片扩散法进行的抗菌生物活性测试表明，松萝酸化合物在浓度为 500mg/mL 和 1000mg/mL 时，对大肠杆菌（ATCC35218）和金黄色葡萄球菌（ATCC25923）的生长具有明显的抑制作用，抑菌圈在 12mm 至 17mm 之间。此外，伤寒沙门氏菌（YCTC）仅在浓度为 1000mg/mL 时被抑制，抑菌圈为 14mm。
