Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Halu Oleo.
Research Center for Chemistry, Indonesian Institute of Sciences.
J Oleo Sci. 2022;71(1):127-134. doi: 10.5650/jos.ess21211.
Lichen Usnea sp. is potential as a new natural medicine. This study report isolation of secondary metabolites from lichen Usnea sp. and α-glucosidase inhibitory, which is potential as an antidiabetic agent. Lichen powder was macerated using methanol, separated using column chromatography gravity and thin-layer chromatography. The crystalline was isolated and purified by the recrystallization process for obtaining pure compound. The isolated compound was determined using FTIR and NMR spectroscopy (H and C). The results showed that the isolated compound was yellow needle crystals. Based on the spectra data interpretation, it was obtained usnic acid compound with the molecular formula of CHO. The α-glucosidase inhibitory activity test showed that the usnic acid had activity in inhibiting the α-glucosidase enzyme with an IC value of 106.78 µg/mL. The usnic acid from Usnea sp. has a very good impact on the source of natural compounds as an antidiabetic drug in the future.
石松属地衣是一种有潜力的天然药物。本研究报告了从石松属地衣中分离出的次生代谢产物，以及具有抗糖尿病作用的α-葡萄糖苷酶抑制活性。地衣粉末用甲醇浸渍，采用柱层析重力和薄层层析进行分离。通过重结晶过程对结晶进行分离和纯化，以获得纯化合物。利用傅里叶变换红外光谱（FTIR）和核磁共振波谱（H 和 C）确定分离得到的化合物。结果表明，分离得到的化合物为黄色针状晶体。根据谱图数据分析，得到了化合物为石松酸，其分子式为 CHO。α-葡萄糖苷酶抑制活性测试表明，石松酸对 α-葡萄糖苷酶具有抑制活性，IC 值为 106.78 µg/mL。石松属地衣中的石松酸作为一种天然化合物来源，对未来的抗糖尿病药物具有很好的应用前景。
