Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Department of Biotechnology and Bioinformatics, International Islamic University, Islamabad, Pakistan.
Pak J Pharm Sci. 2020 Jul;33(4):1671-1677.
The Vitex negeundo is a widely used medicinal plant which has not been fully investigated in the past. We assessed the in vivo hepatoprotective and in vitro antioxidant, antibacterial, cytotoxicity and antiproliferative study of leaf extracts of V. Neugundo. The chemically profiled using HPLC, three flavonoids were quantified and GC-MS analysis revealed the presence of two new compounds those were not reported earlier. The animal study was conducted on mice treated with CCl4 using methanolic and chloroform extracts (100, 200 and 300 mg/kg b.w), with silymarin as a positive control. Hepatoprotective effects were determined by analyzing blood for liver marker enzymes, direct bilirubins and hematological parameters (RBC, WBC and platelets). The methanolic extract (300 mg/kg b.w) has shown the stronger hepatoprotective effects against abnormalities produced by CCl. The in vivo hepatoprotective effects correlated well with the in vitro antioxidant, cytotoxicity and antiproliferative activities and with high levels of flavonoids and other organic compounds analyzed from plant extracts. The leaf extracts of this plant could be good candidates for lead compound required for the development of antioxidant/anticancer drugs.
牡荆是一种被广泛应用的药用植物，但过去对其并未进行充分的研究。我们评估了牡荆叶提取物的体内保肝和体外抗氧化、抗菌、细胞毒性和抗增殖研究。采用高效液相色谱法对化学成分进行了分析，定量了三种类黄酮，并通过 GC-MS 分析发现了两种以前未报道过的新化合物。在使用 CCl4 处理的小鼠上进行了动物研究，采用甲醇和氯仿提取物（100、200 和 300mg/kg b.w），以水飞蓟素作为阳性对照。通过分析血液中的肝标志物酶、直接胆红素和血液学参数（RBC、WBC 和血小板）来确定保肝作用。甲醇提取物（300mg/kg b.w）对 CCl 引起的异常显示出更强的保肝作用。体内保肝作用与体外抗氧化、细胞毒性和抗增殖活性以及从植物提取物中分析出的高水平类黄酮和其他有机化合物密切相关。这种植物的叶提取物可能是开发抗氧化/抗癌药物所需的先导化合物的良好候选物。
