Cao Yaping, Zhao Guodong, Yuan Mufa, Liu Xiaoyu, Ma Yong, Cao Yang, Miao Bei, Zhao Shuyan, Li Danning, Xiong Shangmin, Zheng Minxue, Fei Sujuan
Department of Gastroenterology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Institute of Digestive Diseases, Xuzhou Medical University, Xuzhou, China.
Front Oncol. 2021 Jan 29;10:621295. doi: 10.3389/fonc.2020.621295. eCollection 2020.
Aberrant DNA methylation has emerged as a class of promising biomarkers for early colorectal cancer (CRC) detection, but the performance of methylated and methylated in stool DNA has never been evaluated.
Methylation specific quantitative PCR (qPCR) assays for methylated and methylated were developed. The methylation levels of and in 198 CRC patients, 20 advanced adenoma (AA) patients, 101 small polyp (SP) patients, and 141 no evidence of disease (NED) subjects were analyzed.
The methylation levels of both and genes were significantly higher in CRC and AA groups than those in SP and NED groups, but showed no significant difference among different stages of CRC. The sensitivities of methylated and methylated for CRC detection were 77.3% (95% CI: 70.7-82.8%) and 85.9% (95% CI: 80.0-90.2%) with specificities of 91.5% (95% CI: 85.3-95.3%) and 95.0% (95% CI: 89.7-97.8%), respectively. When and methylated were combined, the clinical performance for CRC detection was similar to that of methylated alone.
Stool DNA based methylated test has the potential to become an alternative approach for CRC screening and prevention.
异常DNA甲基化已成为一类有前景的早期结直肠癌（CRC）检测生物标志物，但粪便DNA中甲基化 和甲基化 的性能从未得到评估。
开发了针对甲基化 和甲基化 的甲基化特异性定量PCR（qPCR）检测方法。分析了198例CRC患者、20例晚期腺瘤（AA）患者、101例小息肉（SP）患者和141例无疾病证据（NED）受试者中 和 的甲基化水平。
CRC组和AA组中 和 基因的甲基化水平均显著高于SP组和NED组，但在CRC不同阶段之间无显著差异。甲基化 和甲基化 检测CRC的敏感性分别为77.3%（95%CI：70.7 - 82.8%）和85.9%（95%CI：80.0 - 90.2%），特异性分别为91.5%（95%CI：85.3 - 95.3%）和95.0%（95%CI：89.7 - 97.8%）。当甲基化 和甲基化 联合使用时，检测CRC的临床性能与单独使用甲基化 相似。
基于粪便DNA的甲基化 检测有潜力成为CRC筛查和预防的替代方法。
