Laboratory of Health Science, Postgraduate Program in Health Science, Department of Health Science, Hospital Universitário Clemente Faria, Universidade Estadual de Montes Claros (Unimontes), Avenida Cula Mangabeira, 562 - Santo Expedito, Montes Claros, Minas Gerais, CEP 39401-001, Brazil.
Instituto Federal de Minas Gerais (IFMG), São João Evangelista, Minas Gerais, Brazil.
Mol Biol Rep. 2021 Feb;48(2):1725-1734. doi: 10.1007/s11033-021-06190-7. Epub 2021 Feb 14.
The present study aimed to evaluate the effects of resveratrol, a nutraceutical polyphenol, and Lactococcus lactis (bacteria probiotic), on metabolic parameters and hepatic proinflammatory markers expression. C57BL/6 mice were divided into 4 groups: Standard (ST), Lactococcus lactis (LL), Resveratrol (RSV), and Lactococcus lactis plus resveratrol (LL + RSV). Lactococcus lactis and resveratrol were administered by orogastric gavage. Blood parameters were assessed (total cholesterol, triglycerides, ALT and AST). IL-6 mRNA expression was evaluated by Real-time PCR and TNF-α protein expression was assessed by immunohistochemistry. The main findings showed that resveratrol and Lactococcus lactis association decreased body weight, aspartate aminotransferase and total cholesterol levels. LL and LL + RSV decreased triglycerides levels and IL-6 and TNF-α expression. These results open a perspective of using resveratrol and Lactococcus lactis to improve metabolic parameters and Lactococcus lactis in preventing inflammation and the hepatic diseases development.
本研究旨在评估白藜芦醇（一种天然多酚）和乳球菌（益生菌）对代谢参数和肝内促炎标志物表达的影响。C57BL/6 小鼠分为 4 组：标准组（ST）、乳球菌组（LL）、白藜芦醇组（RSV）和乳球菌加白藜芦醇组（LL+RSV）。通过口服灌胃给予乳球菌和白藜芦醇。检测血液参数（总胆固醇、甘油三酯、ALT 和 AST）。通过实时 PCR 评估 IL-6 mRNA 表达，通过免疫组织化学评估 TNF-α 蛋白表达。主要发现表明，白藜芦醇和乳球菌的联合使用降低了体重、天冬氨酸转氨酶和总胆固醇水平。LL 和 LL+RSV 降低了甘油三酯水平以及 IL-6 和 TNF-α 的表达。这些结果为使用白藜芦醇和乳球菌来改善代谢参数以及乳球菌预防炎症和肝脏疾病发展提供了新的视角。
