College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning, China.
J Agric Food Chem. 2021 Feb 24;69(7):2076-2086. doi: 10.1021/acs.jafc.0c07357. Epub 2021 Feb 15.
Novel anti-viral natural product ε-poly-l-lysine (ε-PL) produced by is a homopolymer of l-lysine, of which the underlying molecular mode of action remains to be further elucidated. In this study, ε-PL induced significant fragmentation of tobacco mosaic virus (TMV) virions and delayed the systemic infection of TMV-GFP as well as wild-type TMV in plants. ε-PL treatment also markedly inhibited RNA accumulation of TMV in tobacco BY-2 protoplasts. The results of RNA-seq indicated that the agent induced significantly differential expression of genes that are associated with defense response, stress response, autophagy, and ubiquitination. Among them, 15 critical differential expressed genes were selected for real-time quantitative PCR validation. We further demonstrated that ε-PL can induce host defense responses by assessing the activity of several defense-related enzymes in plants. Our results provided valuable insights into molecular anti-viral mode of action for ε-PL, which is expected to be applied as a novel microbial natural product against plant virus diseases.
由产生的新型抗病毒天然产物ε-聚赖氨酸（ε-PL）是赖氨酸的均聚物，其潜在的分子作用机制尚待进一步阐明。在这项研究中，ε-PL 导致烟草花叶病毒（TMV）病毒粒子的显著碎片化，并延迟了 TMV-GFP 以及野生型 TMV 在植物中的系统感染。ε-PL 处理还显著抑制了烟草 BY-2 原生质体中 TMV 的 RNA 积累。RNA-seq 的结果表明，该试剂诱导与防御反应、应激反应、自噬和泛素化相关的基因显著差异表达。其中，选择了 15 个关键差异表达基因进行实时定量 PCR 验证。我们还通过评估植物中几种防御相关酶的活性进一步证明了 ε-PL 可以诱导宿主防御反应。我们的研究结果为 ε-PL 的分子抗病毒作用模式提供了有价值的见解，有望作为一种新型微生物天然产物用于防治植物病毒病。
