Zhou Tao, Liu He, Huang Yuanmin, Wang Zehao, Shan Yuhang, Yue Yan, Xia Zihao, Liang Yue, An Mengnan, Wu Yuanhua
College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China.
J Fungi (Basel). 2021 Sep 30;7(10):821. doi: 10.3390/jof7100821.
Microbial secondary metabolites produced by are applied to control plant diseases. The metabolite, ε-poly--lysine (ε-PL), is a non-toxic food preservative, but the potential application of this compound as a microbial fungicide in agriculture is rarely reported. In this study, the effect and mode of action of ε-PL on two necrotrophic pathogenic fungi, and , were investigated. The results showed that ε-PL effectively inhibited the mycelial growth of and with EC values of 283 μg/mL and 281 μg/mL, respectively. In addition, ε-PL at the dose of 150 and 300 μg/mL reduced sclerotia formation. The results of the RNA-seq and RT-qPCR validation indicated that ε-PL significantly regulated the gene expression of critical differential expressed genes (DEGs) involved in fungal growth, metabolism, pathogenicity, and induced an increase in the expression of the fungal stress responses and the detoxification genes. These results provided new insights for understanding the modes of action of ε-PL on and and improved the sustainable management of these plant diseases.
由 产生的微生物次级代谢产物被用于防治植物病害。该代谢产物ε-聚赖氨酸（ε-PL）是一种无毒的食品防腐剂，但这种化合物作为微生物杀菌剂在农业中的潜在应用鲜有报道。在本研究中，研究了ε-PL对两种坏死营养型致病真菌 和 的作用效果及作用方式。结果表明，ε-PL能有效抑制 和 的菌丝生长，其EC值分别为283μg/mL和281μg/mL。此外，150和300μg/mL剂量的ε-PL减少了 的菌核形成。RNA测序和RT-qPCR验证结果表明，ε-PL显著调节了参与真菌生长、代谢、致病性的关键差异表达基因（DEGs）的基因表达，并诱导真菌应激反应和解毒基因的表达增加。这些结果为理解ε-PL对 和 的作用方式提供了新的见解，并改善了这些植物病害的可持续管理。
