Department of Integrated Applied Life Science, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi, Kofu, Yamanashi, Japan.
Graduate School of Life and Environmental Sciences, University of Yamanashi, Kofu, Yamanashi, Japan.
Biosci Biotechnol Biochem. 2021 Apr 24;85(5):1215-1226. doi: 10.1093/bbb/zbab023.
We examined whether peripheral leukocytes of mice derived from in vitro αMEM-cultured embryos and exhibiting type 2 diabetes had higher expression of inflammatory-related genes associated with the development of atherosclerosis. Also, we examined the impact of a barley diet on inflammatory gene expression. Adult mice were produced by embryo transfer, after culturing two-cell embryos for 48 h in either α minimal essential media (α-MEM) or potassium simplex optimized medium control media. Mice were fed either a barley or rice diet for 10 weeks. Postprandial blood glucose and mRNA levels of several inflammatory genes, including Tnfa and Nox2, in blood leukocytes were significantly higher in MEM mice fed a rice diet compared with control mice. Barley intake reduced expression of S100a8 and Nox2. In summary, MEM mice exhibited postprandial hyperglycemia and peripheral leukocytes with higher expression of genes related to the development of atherosclerosis, and barley intake reduced some gene expression.
我们研究了源自体外 αMEM 培养胚胎并表现出 2 型糖尿病的小鼠外周白细胞是否具有与动脉粥样硬化发展相关的更高炎症相关基因表达。此外，我们还研究了大麦饮食对炎症基因表达的影响。通过胚胎移植产生成年小鼠，将二细胞胚胎在 α 最小必需培养基（αMEM）或钾 simplex 优化培养基对照培养基中培养 48 小时。将小鼠分别用大麦或大米饮食喂养 10 周。与对照组相比，用大米饮食喂养的 MEM 小鼠的餐后血糖和血液白细胞中几种炎症基因（包括 Tnfa 和 Nox2）的 mRNA 水平显著升高。大麦摄入可降低 S100a8 和 Nox2 的表达。总之，MEM 小鼠表现出餐后高血糖和外周白细胞中与动脉粥样硬化发展相关的基因表达升高，而大麦摄入可降低某些基因表达。
