Modulatory effect of pigeon pea-wheat biscuits on lipid profile, lipid peroxidation level, α-glucosidase, and butyrylcholinesterase activities in type-2 diabetic patients.

This study examined the modulatory effect of pigeon pea-wheat biscuits on lipid profile, lipid peroxidation level, α-glucosidase, and butyrylcholinesterase activities in type-2 diabetic patients. Composite flours were formulated using pigeon pea (PP) and wheat flour (WF) in ratio 100:0, 75:25, 50:50, 25:75, and 0:100. Sixty diabetic patients (DP) were treated with biscuit samples and the effect of the biscuits on the serum lipid profile, α-glucosidase, and butyrylcholinesterase (BChE) activities were evaluated. The result revealed that total cholesterol ranged from [100-PP (13.94 ± 1.05 mg/dl)-REF (40.46 ± 3.09 mg/dl)]; triglyceride [100-PP (28.00 ± 1.14)-REF (115.00 ± 7.05)], low-density lipoprotein (LDL-C) [100-PP (0.75 ± 0.30)-REF (13.30 ± 2.04)], high-density lipoprotein level(HDL-C) [REF (4.16 ± 0.49)-100-PP (7.59 ± 1.62)], LDL-C/HDL-C [100-PP (0.098 ± 0.01)-REF (8.72 ± 1.20)]. Alanine aminotransferase [CAB (3.10 ± 0.51 U/L)-REF (6.90 ± 0.84 U/L)]; aspartate aminotransferase [100-PP (9.50 ± 0.61 U/L)-REF (19.75 ± 1.50 U/L)]; alkaline phosphate (ALP) [CAB (26.99 ± 2.13 U/L)-BASELINE (UT) (92.40 ± 5.41 U/L]. α-glucosidase [100-PP (1.20 ± 0.07)-REF (0.37 ± 0.02)] and BChE [100-PP (1.12 ± 0.09)-REF (1.30 ± 0.01)] and Malondialdehyde [100-PP (0.33 ± 0.08)-REF (0.37 ± 0.04)]. 100-PP had an outstanding antihyperlipidemic potentials, not injurious to liver and might serve as good functional food that could help in preventing the risk of developing heart coronary disease, atherosclerosis, and stroke. PRACTICAL APPLICATIONS: This study provides vital information on the possible effect of pigeon pea-wheat biscuits on the serum lipid profile and peroxidation levels, α-glucosidase and butyrylcholinesterase of diabetic patients. The biscuits produced had lowering effect on cholesterol and low-density lipoprotein but improved high-density lipoprotein. All the biscuits have good lipid profile which may lower the risk of developing coronary heart diseases and vascular heart diseases in diabetes. However, 100-PP biscuit had an outstanding antihyperlipidemia action on the serum lipid levels of the patients. Also, it had the highest α-glucosidase, MDA, and BChE inhibitory potential. ALT, AST, and ALP serum activities of the patient's serum were greatly improved after treatment with the biscuits. 100-PP could lower the risk of developing heart coronary disease, atherosclerosis, and stroke.