Institute for Complex Molecular Systems, Eindhoven University of Technology, P. O. Box 513 (STO 3.41), 5600MB Eindhoven, The Netherlands.
Institute for Molecules and Materials, Radboud University, 6525 AJ Nijmegen, The Netherlands.
ACS Appl Mater Interfaces. 2021 Feb 24;13(7):7879-7889. doi: 10.1021/acsami.0c19052. Epub 2021 Feb 15.
Polymer-stabilized complex coacervate microdroplets have emerged as a robust platform for synthetic cell research. Their unique core-shell properties enable the sequestration of high concentrations of biologically relevant macromolecules and their subsequent release through the semipermeable membrane. These unique properties render the synthetic cell platform highly suitable for a range of biomedical applications, as long as its biocompatibility upon interaction with biological cells is ensured. The purpose of this study is to investigate how the structure and formulation of these coacervate-based synthetic cells impact the viability of several different cell lines. Through careful examination of the individual synthetic cell components, it became evident that the presence of free polycation and membrane-forming polymer had to be prevented to ensure cell viability. After closely examining the structure-toxicity relationship, a set of conditions could be found whereby no detrimental effects were observed, when the artificial cells were cocultured with RAW264.7 cells. This opens up a range of possibilities to use this modular system for biomedical applications and creates design rules for the next generation of coacervate-based, biomedically relevant particles.
聚合物稳定的复杂凝聚层微滴已成为合成细胞研究的强大平台。它们独特的核壳特性能够隔离高浓度的生物相关大分子，并通过半透膜进行后续释放。这些独特的特性使得合成细胞平台非常适合一系列生物医学应用，只要其与生物细胞相互作用时具有生物相容性得到保证。本研究旨在探讨这些凝聚层基合成细胞的结构和配方如何影响几种不同细胞系的活力。通过仔细研究各个合成细胞成分，显然必须防止游离聚阳离子和形成膜的聚合物的存在，以确保细胞活力。在仔细研究结构-毒性关系后，可以找到一组条件，即在与 RAW264.7 细胞共培养时，不会观察到人工细胞产生有害影响。这为使用这种模块化系统进行生物医学应用开辟了一系列可能性，并为下一代基于凝聚层的、与生物医学相关的颗粒创造了设计规则。
