神经降压素对幽门结扎大鼠中五肽胃泌素刺激胃酸分泌的抑制作用。

Inhibitory effect of neurotensin on pentagastrin-stimulated gastric acid secretion in pylorus-ligated rats.

作者信息

Hammer R A, Wagner B, Soyode H S

机构信息

Dept. of Medicine, Northwestern Univ. Medical School, Chicago, IL.

出版信息

Biochem Biophys Res Commun. 1988 Apr 15;152(1):231-6. doi: 10.1016/s0006-291x(88)80704-6.

DOI:10.1016/s0006-291x(88)80704-6

PMID:3358763

Abstract

Neurotensin is a known inhibitor of gastrin-stimulated acid secretion in dogs and humans. In order to study the dose-related effect of neurotensin, we prepared pentobarbital-anesthetized rats by pyloric ligation and collected gastric secretions one hour after injection of saline (Basal), pentagastrin, 6 micrograms/Kg subcutaneously (PG Alone), or pentagastrin plus neurotensin by tail vein injection (PG + NT). Acid output was calculated from the volume and pH of the samples, which correlated well with the output determined by titration with 0.02 N NaOH (r = 0.92). Basal output was 36 +/- 4 muEq/hr; stimulated output (PG Alone) was 64 +/- 5 muEq/hr, and output after PG + NT, 250 pmol/Kg, was 33 +/- 3 muEq/hr (p less than 0.001). The effect of neurotensin was dose-related over a range from 125 to 500 pmol/Kg. This technique may be useful in the biological evaluation of neurotensin-related peptides.

摘要

神经降压素是一种已知的对犬类和人类胃泌素刺激胃酸分泌的抑制剂。为了研究神经降压素的剂量相关效应，我们通过幽门结扎制备了戊巴比妥麻醉的大鼠，并在注射生理盐水（基础值）、五肽胃泌素（皮下注射6微克/千克，单独使用五肽胃泌素）或通过尾静脉注射五肽胃泌素加神经降压素（五肽胃泌素+神经降压素）1小时后收集胃分泌物。根据样本的体积和pH计算酸排出量，其与用0.02N氢氧化钠滴定测定的排出量相关性良好（r = 0.92）。基础排出量为36±4微当量/小时；刺激后的排出量（单独使用五肽胃泌素）为64±5微当量/小时，五肽胃泌素+神经降压素（250皮摩尔/千克）后的排出量为33±3微当量/小时（p＜0.001）。在125至500皮摩尔/千克的范围内，神经降压素的作用呈剂量相关。该技术可能有助于神经降压素相关肽的生物学评估。