Will B E, Toniolo G, Brailowsky S
Laboratoire de Neurobiologie des Comportements, Université Louis Pasteur, Strasbourg, France.
Behav Brain Res. 1988 Feb;27(2):123-9. doi: 10.1016/0166-4328(88)90038-1.
Motor behavior was investigated in rats following acute and chronic gamma-aminobutyric acid (GABA) microinfusions into the nucleus basalis. For acute treatment, the rats received GABA (100 micrograms in 1 microliter), then saline, or these solutions in the reverse order, into the nucleus basalis contralateral to their preferred turning direction in a radial maze. For chronic treatment, half the rats received saline (1 microliter/h for 4 days), and than GABA (100 micrograms/microliters/h) for the same period of time ('saline-first' group). In the other half, this sequence was reversed ('GABA-first' group). Acute microinjections of GABA decreased turning towards the non-injected side; chronic treatment enhanced this effect by reversing the preferred turning direction. Return to initial turning direction was observed after acute GABA-injection in both experimental groups, but only in the 'saline-first' group after chronic treatment. The 'GABA-first' group showed gliosis in and around the nucleus basalis area and a reduction of cortical acetyl-cholinesterase-positivity which were significantly greater than in the 'saline-first' group. This, chronic saline pretreatment is associated with diminished neurotoxicity of chronic GABA infusion and with a reversibility of the drug-induced behavioral effects.
在将急性和慢性γ-氨基丁酸（GABA）微量注入基底核后，对大鼠的运动行为进行了研究。对于急性治疗，大鼠在放射状迷宫中，在与它们偏好的转向方向对侧的基底核中，先接受GABA（1微升含100微克），然后是生理盐水，或者将这些溶液的顺序颠倒。对于慢性治疗，一半的大鼠先接受生理盐水（1微升/小时，持续4天），然后在相同时间段内接受GABA（100微克/微升/小时）（“先生理盐水”组）。另一半大鼠则顺序相反（“先GABA”组）。急性微量注射GABA减少了向未注射侧的转向；慢性治疗通过逆转偏好的转向方向增强了这种效果。在两个实验组中，急性注射GABA后都观察到转向方向恢复到初始状态，但慢性治疗后仅在“先生理盐水”组中观察到。“先GABA”组在基底核区域及其周围显示出胶质细胞增生，并且皮质乙酰胆碱酯酶阳性率降低，这明显大于“先生理盐水”组。因此，慢性生理盐水预处理与慢性GABA输注的神经毒性降低以及药物诱导的行为效应的可逆性有关。
