长链非编码RNA LINC00342通过靶向miR-19a-3p/NPEPL1轴促进结直肠癌的生长和转移。
LncRNA LINC00342 contributes to the growth and metastasis of colorectal cancer via targeting miR-19a-3p/NPEPL1 axis.
作者信息
Shen Peng, Qu Lili, Wang Jingjing, Ding Quchen, Zhou Chuanwen, Xie Rui, Wang Honggang, Ji Guozhong
机构信息
The Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210000, China.
The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huai'an, 223300, China.
出版信息
Cancer Cell Int. 2021 Feb 15;21(1):105. doi: 10.1186/s12935-020-01705-x.
BACKGROUND
Long intergenic non-protein coding RNA 00342 (LINC00342) has been identified as a novel oncogene. However, the functional role of LINC00342 in colorectal cancer (CRC) remains unclear.
METHODS
The expression of LINC00342 is detected by real-time PCR (RT-PCR) analysis. Cell proliferation, migration and invasion and xenograft model are examined to analyze the biological functions of LINC00342 in vitro and in vivo using colony formation, would healing and transwell analyses. Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays are used to identify the target interactions between LINC00342, miR-19a-3p and aminopeptidase like 1 (NPEPL1).
RESULTS
LINC00342 was highly expressed in CRC. Down-regulation of LINC00342 inhibited cell proliferation and metastasis of CRC cells. Moreover, knocking down LINC00342 inhibited the tumor growth in vivo. Mechanistic investigation revealed that LINC00342 might sponge miR-19a-3p to regulate NPEPL1 expression. Further investigation indicated that the ontogenesis facilitated by LINC00342 was inhibited due to the depletion of NPEPL1.
CONCLUSION
LINC00342 promotes CRC progression by competitively binding miR-19a-3p with NPEPL1.
背景
长链基因间非编码RNA 00342（LINC00342）已被鉴定为一种新型致癌基因。然而，LINC00342在结直肠癌（CRC）中的功能作用仍不清楚。
方法
通过实时荧光定量PCR（RT-PCR）分析检测LINC00342的表达。使用集落形成、伤口愈合和Transwell分析等方法，检测细胞增殖、迁移和侵袭以及异种移植模型，以分析LINC00342在体外和体内的生物学功能。采用双荧光素酶报告基因和RNA免疫沉淀（RIP）试验，鉴定LINC00342、miR-19a-3p和氨肽酶样1（NPEPL1）之间的靶向相互作用。
结果
LINC00342在CRC中高表达。下调LINC00342可抑制CRC细胞的增殖和转移。此外，敲低LINC00342可抑制体内肿瘤生长。机制研究表明LINC00342可能通过吸附miR-19a-3p来调节NPEPL1的表达。进一步研究表明由于NPEPL1的缺失，LINC00342促进的肿瘤发生受到抑制。
结论
LINC00342通过与NPEPL1竞争性结合miR-19a-3p促进CRC进展。
Zotero 插件