Wu Yuyun, Hao Ningbo, Wang Suming, Yang Xin, Xiao Yufeng, Yang Huan, Yang Shiming, Li Bosheng
Department of Gastroenterology, Xinqiao Hospital, Army Medical University (Third Military Medical University), Chongqing 400037, China.
Department of Gastroenterology, PLA Rocket Force Characteristic Medical Center, Beijing Xinjiekouwai Street, Beijing 100088, China.
J Oncol. 2020 Mar 11;2020:8681361. doi: 10.1155/2020/8681361. eCollection 2020.
Gastric cancer (GC) is one of the most common malignancies worldwide, and the tumor metastasis leads to poor outcomes of GC patients. Long noncoding RNAs (lncRNAs) have emerged as new regulatory molecules that play a crucial role in tumor metastasis. However, the biological function and underlying mechanism of numerous lncRNAs in GC metastasis remain largely unclear. Here, we report a novel lncRNA, lnc-TLN2-4:1, whose expression is decreased in GC tissue versus matched normal tissue, and its low expression is involved in the lymph node and distant metastases of GC, as well as poor overall survival rates of GC patients. We further found that lnc-TLN2-4:1 inhibits the ability of GC cells to migrate and invade but does not influence GC cell proliferation and confirmed that lnc-TLN2-4:1 is mainly located in the cytoplasm of GC cells. We then found that lnc-TLN2-4:1 increases the mRNA and protein expression of TLN2 in GC cells and there is a positive correlation between the expression of lnc-TLN2-4:1 and TLN2 mRNA in GC tissue. Collectively, we identified a novel lncRNA, lnc-TLN2-4:1, in GC, where lnc-TLN2-4:1 represses cell migration and invasion. The low expression of lnc-TLN2-4:1 is associated with poor overall survival rates of GC patients. These suggest that lnc-TLN2-4:1 may be a tumor suppressor during GC metastasis.
胃癌（GC）是全球最常见的恶性肿瘤之一，肿瘤转移导致GC患者预后不良。长链非编码RNA（lncRNAs）已成为在肿瘤转移中起关键作用的新型调控分子。然而，众多lncRNAs在GC转移中的生物学功能和潜在机制仍不清楚。在此，我们报道了一种新型lncRNA，lnc-TLN2-4:1，其在GC组织中的表达相对于匹配的正常组织降低，其低表达与GC的淋巴结转移和远处转移以及GC患者较差的总生存率有关。我们进一步发现lnc-TLN2-4:1抑制GC细胞的迁移和侵袭能力，但不影响GC细胞增殖，并证实lnc-TLN2-4:1主要位于GC细胞的细胞质中。然后我们发现lnc-TLN2-4:1增加GC细胞中TLN2的mRNA和蛋白表达，并且lnc-TLN2-4:1与GC组织中TLN2 mRNA的表达之间存在正相关。总体而言，我们在GC中鉴定出一种新型lncRNA，lnc-TLN2-4:1，其中lnc-TLN2-4:1抑制细胞迁移和侵袭。lnc-TLN2-4:1的低表达与GC患者较差的总生存率相关。这些表明lnc-TLN2-4:1可能是GC转移过程中的一种肿瘤抑制因子。
