[长链非编码RNA LINC00342通过靶向miR-596调控胃癌细胞的增殖、迁移和侵袭]
[LncRNA LINC00342 regulates gastric cancer cell proliferation, migration and invasion by targeting miR-596].
作者信息
Xin B, Liu G, Zhang C, Wang B, Shi L
机构信息
Department of Gastrointestinal Surgery, Affiliated Yijishan Hospital of Wannan Medical College, Wuhu 241001, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2023 Oct 20;43(10):1761-1770. doi: 10.12122/j.issn.1673-4254.2023.10.14.
OBJECTIVE
To investigate the expression levels of LINC00342 in gastric cancer (GC) tissues and cells and the pathways mediating its effects on biological behaviors of GC cells.
METHODS
Bioinformatic analysis was performed to identify the lncRNAs and their downstream miRNAs involved in regulation of biological behaviors of GC cells. qRT-PCR was used to analyze the differential expression of LINC00342 and miR-596 in GC cell lines, human gastric mucosal cells, and GC and adjacent tissues. In human GC MGC-803 and MGC-823 cells, the effects of LINC00342 overexpression, miR-596 overexpression, LINC00342 knockdown, or miR-596 knockdown on cell proliferation, migration, invasion and cell cycle changes were examined using Edu assay, CCK-8 assay, wound healing assay, Transwell assay, and flow cytometry. The regulatory interaction between LINC00342 and miR-596 was investigated using a dual-luciferase reporter assay.
RESULTS
Informatic analysis identified LINC00342 as the candidate lncRNA regulating biological behaviors of GC cells, with miR-596 as its downstream miRNA. LINC00342 expression levels were significantly higher while miR-596 expression levels were lower in GC tissues and cell lines than in the paired adjacent tissues and human gastric mucosal cell lines (all <0.05). In MGC-803 and MGC-823 cells, overexpression of LINC00342 significantly enhanced cell proliferation (<0.05), migration (<0.01), and invasion (<0.001) and reduced the percentage of G0/G1 phase cells (<0.01), while knocking down LINC00342 significantly suppressed cell proliferation (<0.05), migration (<0.01), and invasion (<0.001) and increased G0/G1 phase cell percentage (<0.01). Modulation of miR-596 expression levels produced the opposite effects. Dual-luciferase reporter assay confirmed the specific binding between LINC00342 and miR-596 (=0.0067).
CONCLUSION
In GC cells, LINC00342 regulates cell proliferation, migration, and invasion by targeting miR-596.
目的
探讨LINC00342在胃癌（GC）组织和细胞中的表达水平及其影响GC细胞生物学行为的作用途径。
方法
进行生物信息学分析，以鉴定参与调控GC细胞生物学行为的lncRNAs及其下游miRNAs。采用qRT-PCR分析LINC00342和miR-596在GC细胞系、人胃黏膜细胞以及GC组织和癌旁组织中的差异表达。在人GC MGC-803和MGC-823细胞中，通过Edu检测、CCK-8检测、伤口愈合检测、Transwell检测和流式细胞术，研究LINC00342过表达、miR-596过表达、LINC00342敲低或miR-596敲低对细胞增殖、迁移、侵袭及细胞周期变化的影响。采用双荧光素酶报告基因检测法研究LINC00342与miR-596之间的调控相互作用。
结果
生物信息学分析确定LINC00342为调控GC细胞生物学行为的候选lncRNA，miR-596为其下游miRNA。与配对的癌旁组织和人胃黏膜细胞系相比，GC组织和细胞系中LINC00342表达水平显著升高，而miR-596表达水平降低（均P<0.05）。在MGC-803和MGC-823细胞中，LINC00342过表达显著增强细胞增殖（P<0.05）、迁移（P<0.01）和侵袭（P<0.001），并降低G0/G1期细胞百分比（P<0.01）；而敲低LINC00342则显著抑制细胞增殖（P<0.05）、迁移（P<0.01）和侵袭（P<0.001），并增加G0/G1期细胞百分比（P<0.01）。miR-596表达水平的调节产生相反的效果。双荧光素酶报告基因检测证实LINC00342与miR-596之间存在特异性结合（P=0.006）。
结论
在GC细胞中，LINC00342通过靶向miR-596调控细胞增殖、迁移和侵袭。
Zotero 插件