γδT 细胞在感染伯氏疟原虫的 C57BL/6 小鼠肺部的特征。
Characterization of γδT cells in lung of Plasmodium yoelii-infected C57BL/6 mice.
机构信息
Key Laboratory of Immunology, State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affliated Hospital of Guangzhou Medical University, Guangzhou, 510120, China.
Biological Resource Center, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China.
出版信息
Malar J. 2021 Feb 15;20(1):89. doi: 10.1186/s12936-021-03619-z.
BACKGROUND
Malaria has high morbidity and mortality rates in some parts of tropical and subtropical countries. Besides respiratory and metabolic function, lung plays a role in immune system. γδT cells have multiple functions in producing cytokines and chemokines, regulating the immune response by interacting with other cells. It remains unclear about the role of γδT cells in the lung of mice infected by malaria parasites.
METHODS
Flow cytometry (FCM) was used to evaluate the frequency of γδT cells and the effects of γδT cells on the phenotype and function of B and T cells in Plasmodium yoelii-infected wild-type (WT) or γδTCR knockout (γδT KO) mice. Haematoxylin-eosin (HE) staining was used to observe the pathological changes in the lungs.
RESULTS
The percentage and absolute number of γδT cells in the lung increased after Plasmodium infection (p < 0.01). More γδT cells were expressing CD80, CD11b, or PD-1 post-infection (p < 0.05), while less γδT cells were expressing CD34, CD62L, and CD127 post-infection (p < 0.05). The percentages of IL-4, IL-5, IL-6, IL-21, IL-1α, and IL-17 γδT cells were increased (p < 0.05), but the percentage of IFN-γ-expressing γδT cells decreased (p < 0.05) post-infection. The pathological changes in the lungs of the infected γδT KO mice were not obvious compared with the infected WT mice. The proportion of CD3 cells and absolute numbers of CD3 cells, CD3 CD4 cells, CD3 CD8 cells decreased in γδT KO infected mice (p < 0.05). γδT KO infected mice exhibited no significant difference in the surface molecular expression of T cells compared with the WT infected mice (p > 0.05). While, the percentage of IFN-γ-expressing CD3 and CD3 CD8 cells increased in γδT KO infected mice (p < 0.05). There was no significant difference in the absolute numbers of the total, CD69, ICOS, and CD80 B cells between the WT infected and γδT KO infected mice (p > 0.05).
CONCLUSIONS
The content, phenotype, and function of γδT cells in the lung of C57BL/6 mice were changed after Plasmodium infection. γδT cells contribute to T cell immune response in the progress of Plasmodium infection.
背景
疟疾在一些热带和亚热带国家的发病率和死亡率很高。除了呼吸和代谢功能外，肺在免疫系统中也发挥作用。γδT 细胞在产生细胞因子和趋化因子方面具有多种功能，通过与其他细胞相互作用来调节免疫反应。γδT 细胞在疟原虫感染的小鼠肺部中的作用尚不清楚。
方法
采用流式细胞术（FCM）评估γδT 细胞的频率以及 γδT 细胞对 Plasmodium yoelii 感染的野生型（WT）或 γδTCR 敲除（γδT KO）小鼠中 B 和 T 细胞表型和功能的影响。苏木精-伊红（HE）染色观察肺的病理变化。
结果
疟原虫感染后，肺中γδT 细胞的百分比和绝对数量增加（p<0.01）。感染后，更多的γδT 细胞表达 CD80、CD11b 或 PD-1（p<0.05），而更少的γδT 细胞表达 CD34、CD62L 和 CD127（p<0.05）。IL-4、IL-5、IL-6、IL-21、IL-1α 和 IL-17 γδT 细胞的百分比增加（p<0.05），但 IFN-γ 表达的 γδT 细胞的百分比减少（p<0.05）。与感染 WT 小鼠相比，感染 γδT KO 小鼠的肺部病理变化不明显。γδT KO 感染小鼠的 CD3 细胞比例和 CD3 细胞、CD3 CD4 细胞、CD3 CD8 细胞的绝对数量减少（p<0.05）。与感染 WT 小鼠相比，γδT KO 感染小鼠的 T 细胞表面分子表达没有显著差异（p>0.05）。然而，在感染 γδT KO 的小鼠中，IFN-γ 表达的 CD3 和 CD3 CD8 细胞的百分比增加（p<0.05）。WT 感染和 γδT KO 感染小鼠的总、CD69、ICOS 和 CD80 B 细胞的绝对数量没有显著差异（p>0.05）。
结论
C57BL/6 小鼠感染疟原虫后，肺中 γδT 细胞的含量、表型和功能发生改变。γδT 细胞有助于疟原虫感染过程中的 T 细胞免疫反应。
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