Hong C B, Loomis W F
Department of Biology, University of California San Diego, La Jolla 92093.
Biochim Biophys Acta. 1988 May 6;950(1):61-6. doi: 10.1016/0167-4781(88)90073-5.
The accumulation of mRNA recognized by oligonucleotides coding for a portion of the spore coat protein, SP60, was determined throughout development of Dictyostelium discoideum. The 1.8 kb mRNA first appears at the tipped aggregate stage and accumulates until culmination. This mRNA is present in pre-spore cells but absent from pre-stalk cells. A cDNA clone was selected by the oligonucleotides and found to be homologous to this mRNA. Although the oligonucleotides were designed to match the sequence coding for a hexapeptide repeat at the amino-terminus of SP60, they were able to recognize a similar repeated region at the carboxy-terminus of the protein coded by the cDNA clone. The SP60 gene appears to be subject to temporal and cell-type-specific transcriptional controls that are coordinate with those of SP96, another spore coat gene.
在盘基网柄菌整个发育过程中，测定了编码孢子外壁蛋白SP60一部分的寡核苷酸所识别的mRNA的积累情况。1.8 kb的mRNA最早出现在顶端聚集期，并一直积累到发育成熟。这种mRNA存在于前孢子细胞中，但在前柄细胞中不存在。通过寡核苷酸筛选出一个cDNA克隆，发现它与这种mRNA同源。尽管这些寡核苷酸设计用于匹配SP60氨基末端六肽重复序列的编码序列，但它们能够识别由cDNA克隆编码的蛋白质羧基末端的类似重复区域。SP60基因似乎受到时间和细胞类型特异性转录调控，这些调控与另一个孢子外壁基因SP96的调控协同进行。
