盘基网柄菌孢子外壁蛋白（sp96）基因的分离与鉴定

Isolation and characterization of spore coat protein (sp96) gene of Dictyostelium discoideum.

作者信息

Tasaka M, Hasegawa M, Ozaki T, Iwabuchi M, Takeuchi I

机构信息

Division of Developmental Biology, National Institute for Basic Biology, Okazaki, Japan.

出版信息

Cell Differ Dev. 1990 Jul;31(1):1-9. doi: 10.1016/0922-3371(90)90085-b.

DOI:10.1016/0922-3371(90)90085-b

PMID:1977501

Abstract

A cDNA library was constructed from poly(A)+ RNA isolated from slug cells of Dictyostelium discoideum, using lambda gt11 phage, and screened with an antiserum specific for the spore coat protein sp96. A positive clone was obtained and the gene product was identified as sp96. The sp96 mRNA is 2.2 kb in size, and it starts to accumulate at the tipped aggregate stage only in prespore cells. Southern analysis using nuclear DNA established that the sp96 gene is unique. Two genomic clones containing the sp96 gene were isolated and the sequence of the gene established. The coding region contains a long open reading frame interrupted by a single intron.

摘要

从盘基网柄菌蛞蝓细胞中分离出的聚腺苷酸加尾RNA构建了一个cDNA文库，使用λgt11噬菌体，并以针对孢子壁蛋白sp96的抗血清进行筛选。获得了一个阳性克隆，并将该基因产物鉴定为sp96。sp96 mRNA大小为2.2 kb，仅在芽孢前体细胞中于顶端聚集体阶段开始积累。使用核DNA进行的Southern分析表明sp96基因是唯一的。分离出了两个包含sp96基因的基因组克隆，并确定了该基因的序列。编码区包含一个长的开放阅读框，被一个单一内含子打断。