Gava Danielle, Caron Luizinho, Schaefer Rejane, Silva Virgínia Santiago, Weiblen Rudi, Flores Eduardo Furtado, de Lima Marcelo, Takeda Guilherme Zaha, Ciacci-Zanella Janice Reis
Embrapa Suínos e Aves, Concórdia, Brazil.
Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, Brazil.
Transbound Emerg Dis. 2022 Mar;69(2):903-907. doi: 10.1111/tbed.14036. Epub 2021 Mar 9.
Porcine reproductive and respiratory syndrome (PRRS) is a viral disease characterized by reproductive impairment or failure in breeding animals, and a respiratory disease in pigs of any age. Brazil is the fourth largest pork producer and exporter globally, and PRRS virus (PRRSV) infection has never been reported in the country. This study aimed to investigate the status of porcine biological samples from commercial swine herds, quarantined imported boars, wild boars and feral pigs to update PRRS information in Brazil. A total of 14,382 samples were collected from 2008 to 2020, including sera (n = 12,841), plasma (n = 1,000) and oral fluids (n = 541), comprehending 137 herds and free-living pigs in eight Brazilian states. One out of 1,000 (0.1%) plasma and 15 out of 12,841 (0.11%) serum samples tested positive for PRRSV antibodies through ELISA. Upon ELISA retesting, only the plasma sample, from one 8-day-old piglet remained positive. All sixteen previously PRRSV antibody-positive samples were tested through RT-PCR and found to be negative. The presence of false-positive or singleton reactors are quite expected. Thus, the use of different/alternative diagnostic tests is indicated for an efficient PRRSV detection. Taken together, our findings demonstrated no conclusive evidence of PRRSV infection in the tested pigs, highlighting the importance to reinforce the surveillance program to prevent the introduction and eventual dissemination of PRRSV in Brazil.
猪繁殖与呼吸综合征（PRRS）是一种病毒性疾病，其特征是繁殖动物出现繁殖障碍或繁殖失败，以及任何年龄的猪出现呼吸道疾病。巴西是全球第四大猪肉生产国和出口国，该国从未报告过猪繁殖与呼吸综合征病毒（PRRSV）感染情况。本研究旨在调查来自商业猪群、隔离进口公猪、野猪和野猪的猪生物样本状况，以更新巴西的PRRS信息。2008年至2020年共采集了14382份样本，包括血清（n = 12841）、血浆（n = 1000）和口腔液（n = 541），涵盖巴西八个州的137个猪群和散养猪。通过ELISA检测，1000份血浆样本中有1份（0.1%）和12841份血清样本中有15份（0.11%）PRRSV抗体检测呈阳性。ELISA复测时，只有一份来自8日龄仔猪的血浆样本仍呈阳性。所有之前PRRSV抗体呈阳性的16份样本均通过RT-PCR检测，结果为阴性。出现假阳性或单个反应阳性是很常见的。因此，建议使用不同的/替代诊断测试来进行有效的PRRSV检测。综上所述，我们的研究结果表明，在检测的猪中没有PRRSV感染的确凿证据，这突出了加强监测计划以防止PRRSV在巴西引入和最终传播的重要性。
