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多价病毒样流感疫苗可诱导猪产生广泛的细胞和体液免疫。

A polyvalent virosomal influenza vaccine induces broad cellular and humoral immunity in pigs.

机构信息

Laboratório de Virologia, Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600, Porto Alegre, Rio Grande Do Sul, CEP 90035-003, Brazil.

Embrapa Suínos e Aves, BR-153, Km 110, Concórdia, Santa Catarina, CEP 89715-899, Brazil.

出版信息

Virol J. 2023 Aug 16;20(1):181. doi: 10.1186/s12985-023-02153-5.

DOI:10.1186/s12985-023-02153-5
PMID:37587490
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10428566/
Abstract

BACKGROUND

Influenza A virus (IAV) is endemic in pigs globally and co-circulation of genetically and antigenically diverse virus lineages of subtypes H1N1, H1N2 and H3N2 is a challenge for the development of effective vaccines. Virosomes are virus-like particles that mimic virus infection and have proven to be a successful vaccine platform against several animal and human viruses.

METHODS

This study evaluated the immunogenicity of a virosome-based influenza vaccine containing the surface glycoproteins of H1N1 pandemic, H1N2 and H3N2 in pigs.

RESULTS

A robust humoral and cellular immune response was induced against the three IAV subtypes in pigs after two vaccine doses. The influenza virosome vaccine elicited hemagglutinin-specific antibodies and virus-neutralizing activity. Furthermore, it induced a significant maturation of macrophages, and proliferation of B lymphocytes, effector and central memory CD4 and CD8 T cells, and CD8 T lymphocytes producing interferon-γ. Also, the vaccine demonstrated potential to confer long-lasting immunity until the market age of pigs and proved to be safe and non-cytotoxic to pigs.

CONCLUSIONS

This virosome platform allows flexibility to adjust the vaccine content to reflect the diversity of circulating IAVs in swine in Brazil. The vaccination of pigs may reduce the impact of the disease on swine production and the risk of swine-to-human transmission.

摘要

背景

甲型流感病毒（IAV）在全球范围内流行于猪群中，遗传和抗原上多样化的亚型 H1N1、H1N2 和 H3N2 病毒谱系的共同流行对有效疫苗的开发构成了挑战。类病毒颗粒是模拟病毒感染的病毒样颗粒，已被证明是针对多种动物和人类病毒的成功疫苗平台。

方法

本研究评估了含有 H1N1 大流行、H1N2 和 H3N2 三种 IAV 表面糖蛋白的类病毒流感疫苗在猪中的免疫原性。

结果

在两次疫苗接种后，猪对三种 IAV 亚型产生了强大的体液和细胞免疫应答。流感类病毒疫苗诱导了针对血凝素的特异性抗体和病毒中和活性。此外，它还诱导了巨噬细胞的显著成熟，以及 B 淋巴细胞、效应和中央记忆 CD4 和 CD8 T 细胞以及产生干扰素-γ的 CD8 T 淋巴细胞的增殖。此外，该疫苗具有在猪达到市场年龄时提供持久免疫力的潜力，并被证明对猪是安全且无细胞毒性的。

结论

该类病毒平台允许灵活调整疫苗内容，以反映巴西猪群中循环 IAV 的多样性。对猪进行疫苗接种可能会降低疾病对猪生产的影响以及猪与人之间传播的风险。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a65f/10428566/c5ae4b4cdeb8/12985_2023_2153_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a65f/10428566/2f90a25a5656/12985_2023_2153_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a65f/10428566/dd07bb52b960/12985_2023_2153_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a65f/10428566/cb5611a397b5/12985_2023_2153_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a65f/10428566/f20490c4d511/12985_2023_2153_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a65f/10428566/f21d97c33561/12985_2023_2153_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a65f/10428566/c5ae4b4cdeb8/12985_2023_2153_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a65f/10428566/2f90a25a5656/12985_2023_2153_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a65f/10428566/dd07bb52b960/12985_2023_2153_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a65f/10428566/cb5611a397b5/12985_2023_2153_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a65f/10428566/f20490c4d511/12985_2023_2153_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a65f/10428566/f21d97c33561/12985_2023_2153_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a65f/10428566/c5ae4b4cdeb8/12985_2023_2153_Fig6_HTML.jpg

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