Suchy S F, Yamamoto M, Barbero L, Schwarting G A
Department of Biochemistry, Eunice Kennedy Shriver Center for Mental Retardation, Inc., Waltham, MA 02254.
Brain Res. 1988 Feb 2;440(1):25-34. doi: 10.1016/0006-8993(88)91155-9.
A monoclonal antibody, WCC4, raised against PC12 cells, recognizes a ganglioside which is present in low concentrations in the postnatal rat nervous system. The antigen is also present in the adrenal and kidney, as determined immunohistochemically, but is not detectable in liver or spleen. A neutral glycosphingolipid is also immunoreactive. In the present report, the chemical characterization of this ganglioside, isolated from PC12 cells, and the anatomical distribution of the antigens recognized by the WCC4 antibody are described. By enzymatic cleavage of terminal saccharide moieties, the ganglioside is identified as alpha-galactosyl, (alpha-fucosyl) GM1. The ganglioside increases in concentration postnatally to day 35 (P35) and is present in a slightly diminished concentration in the adult. Immunohistochemical studies revealed that this glycolipid is also present on neuronal cell soma throughout the cerebrum, cerebellum and spinal cord. It is expressed in highest concentration in the molecular layer of the dentate gyrus and is also present in the olfactory bulb, the molecular layer of the hippocampus, the piriform cortex, the olfactory tubercle and the entorhinal cortex. The dentate molecular layer receives most of its innervation from neurons in the entorhinal cortex, and gangliosides are known to have an effect on plasticity following entorhinal cortical lesions. Therefore, the WCC4 antibody should prove to be a useful tool for the study of the role of endogenous gangliosides in this region of the nervous system.
一种针对PC12细胞产生的单克隆抗体WCC4，可识别一种神经节苷脂，该神经节苷脂在新生大鼠神经系统中的浓度较低。免疫组织化学检测表明，该抗原也存在于肾上腺和肾脏中，但在肝脏或脾脏中未检测到。一种中性糖鞘脂也具有免疫反应性。在本报告中，描述了从PC12细胞中分离出的这种神经节苷脂的化学特性，以及WCC4抗体识别的抗原的解剖分布。通过对末端糖基部分的酶切，该神经节苷脂被鉴定为α-半乳糖基，（α-岩藻糖基）GM1。该神经节苷脂在出生后至第35天（P35）浓度升高，在成年期浓度略有降低。免疫组织化学研究表明，这种糖脂也存在于整个大脑、小脑和脊髓的神经元细胞体上。它在齿状回分子层中的表达浓度最高，也存在于嗅球、海马分子层、梨状皮质、嗅结节和内嗅皮质中。齿状分子层大部分神经支配来自内嗅皮质的神经元，已知神经节苷脂对内嗅皮质损伤后的可塑性有影响。因此，WCC4抗体应被证明是研究内源性神经节苷脂在该神经系统区域作用的有用工具。
