Schwarting G A, Gajewski A, Barbero L, Tischler A S, Costopoulos D
Neuroscience. 1986 Oct;19(2):647-56. doi: 10.1016/0306-4522(86)90287-3.
The effect of nerve growth factor treatment on the expression of neutral glycosphingolipids and gangliosides was examined in PC12 pheochromocytoma cells grown in spinner culture. These studies show that PC12 cells contain an unusual group of fucose containing neutral glycolipids and gangliosides. The fucose containing neutral glycolipids, which appear to be derivatives of globoside, are very minor components. However, nearly all of the gangliosides in PC12 cells contain fucose. Two minor neutral glycolipids were isolated from these cells, and were identified as fucosylgloboside and galactosylgloboside. Two major gangliosides were also isolated and identified as fucosyl GM2 and fucosyl GD2. Studies using [3H]fucose to examine the synthesis of fucosylglycolipids indicate that nerve growth factor enhances the incorporation of fucose into glycolipids and gangliosides by as much as 80%. These studies show that all of the gangliosides in PC12 cells appear to increase in concentration when the cells are treated with nerve growth factor in spinner culture. However, only the complex fucose containing neutral glycolipids increase in concentration during this same treatment, while the non-fucosylated precursors do not.
在旋转培养中生长的PC12嗜铬细胞瘤细胞中，研究了神经生长因子处理对中性糖鞘脂和神经节苷脂表达的影响。这些研究表明，PC12细胞含有一组不同寻常的含岩藻糖的中性糖脂和神经节苷脂。含岩藻糖的中性糖脂似乎是球蛋白苷的衍生物，是非常次要的成分。然而，PC12细胞中几乎所有的神经节苷脂都含有岩藻糖。从这些细胞中分离出两种次要的中性糖脂，并鉴定为岩藻糖基球蛋白苷和半乳糖基球蛋白苷。还分离出两种主要的神经节苷脂，并鉴定为岩藻糖基GM2和岩藻糖基GD2。使用[3H]岩藻糖研究岩藻糖基糖脂合成的结果表明，神经生长因子可使岩藻糖掺入糖脂和神经节苷脂的量增加多达80%。这些研究表明，在旋转培养中用神经生长因子处理细胞时，PC12细胞中所有神经节苷脂的浓度似乎都会增加。然而，在相同处理过程中，只有复杂的含岩藻糖中性糖脂的浓度增加，而未糖基化的前体则不会。
