Goryacheva Olga A, Beloglazova Natalia V, Goryacheva Irina Yu, De Saeger Sarah
Ghent University, Faculty of Pharmaceutical Sciences, Centre of Excellence in Mycotoxicology and Public Health, Ottergemsesteenweg 460, 9000, Ghent, Belgium; Saratov State University, Chemistry Institute, Department of General and Inorganic Chemistry, Astrakhanskaya 83, 410012, Saratov, Russia.
Ghent University, Faculty of Pharmaceutical Sciences, Centre of Excellence in Mycotoxicology and Public Health, Ottergemsesteenweg 460, 9000, Ghent, Belgium; Nanotechnology Education and Research Center, South Ural State University, Prospect Lenina 76, 485400, Chelyabinsk, Russia.
Talanta. 2021 Apr 1;225:121973. doi: 10.1016/j.talanta.2020.121973. Epub 2020 Dec 13.
Semiconductor quantum dots (QDs) are one of the most popular luminescent labels that are widely used in food and medical analysis. Their unique optical properties establish QDs as excellent tools for highly sensitive biosensors based on Förster resonance energy transfer (FRET). To provide a convenient analytical system with long-term optical stability, a FRET pair consisting of QDs as energy donor and gold nanoparticles (GNs) as energy acceptor was developed. Careful selection of donor and acceptor properties allowed to achieve a large Förster distance of 12.9 nm and to use full-size specific antibody. As the immunoreagents pair, mycotoxins were bound to proteins and then to GNs, while QDs were conjugated with specific antibodies. FRET was observed as a result of the immunocomplex formation. Contributions of FRET and inner filter effect on the quenching were evaluated separately. The quenching effect in the donor-acceptor pair was compared for proteins with different sizes. The developed homogeneous FRET-based immunoassay for the detection of deoxynivalenol (DON) is an example of a fast method for high-throughput control of mycotoxins. The quenching effect of FRET was observed with a limit of detection of 28 μg kg of DON in spiked wheat samples.
半导体量子点（QDs）是最受欢迎的发光标记物之一，广泛应用于食品和医学分析领域。其独特的光学性质使量子点成为基于Förster共振能量转移（FRET）的高灵敏度生物传感器的优良工具。为了提供一个具有长期光学稳定性的便捷分析系统，开发了一种以量子点作为能量供体和金纳米颗粒（GNs）作为能量受体的FRET对。通过仔细选择供体和受体的性质，实现了12.9纳米的大Förster距离，并能够使用全尺寸特异性抗体。作为免疫试剂对，霉菌毒素先与蛋白质结合，然后与GNs结合，而量子点则与特异性抗体偶联。免疫复合物形成后可观察到FRET现象。分别评估了FRET和内滤效应在淬灭过程中的作用。比较了供体-受体对中不同大小蛋白质的淬灭效果。所开发的基于FRET的用于检测脱氧雪腐镰刀菌烯醇（DON）的均相免疫测定法，是一种用于霉菌毒素高通量控制的快速方法。在加标小麦样品中观察到FRET的淬灭效应，DON的检测限为28微克/千克。
