Poshtehban A, Shojaee S, Keshavarz H, Salimi M, Mohebali M
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Trop Biomed. 2017 Jun 1;34(2):433-436.
Toxoplasmosis is an infectious disease caused by the coccidian parasite Toxoplasma gondii. Diagnosis is based on serological methods with detection of specific IgG and IgM antibodies. The present study was performed to compare the sensitivity and specificity of soluble antigen of T. gondii, RH strain obtained from mice and cell culture in ELISA method. Tachyzoites of T. gondii, RH strain that inoculated in mice peritoneum were collected. At the same time, tachyzoites were harvested from HeLa cell culture that infected with the parasite. Soluble antigen was prepared and ELISA method performed on 100 serum samples that were collected from different laboratories in Tehran, Iran. Commercial Trinity kit was used as gold standard. The sensitivity and specificity of T.gondii soluble antigen were higher in antigens that obtained from cell culture in comparison with mice peritoneum. T. gondii cell culture derived antigen has high sensitivity and specificity in ELISA test.
弓形虫病是一种由球虫寄生虫刚地弓形虫引起的传染病。诊断基于检测特异性IgG和IgM抗体的血清学方法。本研究旨在比较从感染小鼠腹水中获得的刚地弓形虫RH株可溶性抗原与细胞培养物中获得的可溶性抗原在ELISA法中的敏感性和特异性。收集接种于小鼠腹腔的刚地弓形虫RH株速殖子。同时，从感染该寄生虫的HeLa细胞培养物中收获速殖子。制备可溶性抗原，并对从伊朗德黑兰不同实验室收集的100份血清样本进行ELISA检测。使用商业Trinity试剂盒作为金标准。与小鼠腹水来源的抗原相比，细胞培养物来源的刚地弓形虫可溶性抗原的敏感性和特异性更高。刚地弓形虫细胞培养物来源的抗原在ELISA检测中具有较高的敏感性和特异性。
