School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, China.
School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, China.
Carbohydr Polym. 2021 Apr 15;258:117657. doi: 10.1016/j.carbpol.2021.117657. Epub 2021 Jan 20.
The present study explored the beneficial effect of Dendrobium huoshanense stem polysaccharide (cDHPS) after oral administration on rheumatoid arthritis (RA) using type Ⅱ collagen-induced arthritis (CIA) mouse model. It was found that cDHPS effectively alleviated joint swelling, synovial hyperplasia, pannus formation, cartilage erosion and bone destruction in CIA mice. Concurrently, cDHPS remodeled the balance of Th17 and regulatory T cells, reduced the secretion of pro-inflammatory mediators related to fibroblast-like synoviocyte activation, angiogenesis, articular cartilage degradation and osteoclast differentiation, inhibited HIF-1α expression and promoted anti-inflammatory mediator release in the joint tissues and serum of CIA mice. Western blot of joint tissues showed that cDHPS significantly inhibited the phosphorylation of IκB, p65, JNK, p38, ERK1/2, AKT, PI3K, JAK1 and STAT3 in CIA mice. These results suggest that cDHPS possesses the potential of ameliorating RA and its anti-RA effect may be attributed to the inhibition of inflammatory signaling pathways.
本研究采用Ⅱ型胶原诱导关节炎（CIA）小鼠模型，探讨了铁皮石斛茎多糖（cDHPS）经口服给药对类风湿关节炎（RA）的有益作用。结果发现，cDHPS 能有效缓解 CIA 小鼠的关节肿胀、滑膜增生、血管翳形成、软骨侵蚀和骨破坏。同时，cDHPS 重塑了 Th17 和调节性 T 细胞的平衡，减少了与成纤维样滑膜细胞激活、血管生成、关节软骨降解和破骨细胞分化相关的促炎介质的分泌，抑制了 CIA 小鼠关节组织和血清中 HIF-1α 的表达，促进了抗炎介质的释放。关节组织的 Western blot 结果表明，cDHPS 显著抑制了 CIA 小鼠中 IκB、p65、JNK、p38、ERK1/2、AKT、PI3K、JAK1 和 STAT3 的磷酸化。这些结果表明，cDHPS 具有改善 RA 的潜力，其抗 RA 作用可能归因于抑制炎症信号通路。
