Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Carbohydr Polym. 2021 Apr 15;258:117680. doi: 10.1016/j.carbpol.2021.117680. Epub 2021 Jan 22.
The bioactive glasses (BGs)/Cisplatin and magnetic bioactive glasses (MBGs)/Cisplatin were doped into the chitosan (CS)-grafted- poly (ε-caprolactone) (PCL) nanofibers for controlled release of Cisplatin under various pH values and temperatures. The simultaneous effect of chemotherapy and hyperthermia was investigated against MG-63 osteosarcoma cells by treating of cells with Cs-g-PCL/MBGs/Cisplatin under an alternating magnetic field. The synthesized nanofibers were characterized using XRD, FTIR, H NMR, SEM, and EDX analysis. The bioactivity, and drug loading efficiency of fibers were investigated. There was no initial burst release of Cisplatin from BGs/Cisplatin and MBGs/Cisplatin loaded Cs-g-PCL/MBGs nanofibers and the Cisplatin release rate was accelerated under pH of 5.5 and temperature of 43 °C compared with physiological condition. The apoptotic/necrotic effect indicated that 100 μg mL nanofibers was optimum for killing of MG-63 cells. The future researches could be focused on the application of nanofibers as an implantable device next to a bone tumor for bone cancer therapy in vivo.
载顺铂的生物活性玻璃（BGs）/Cisplatin 和载顺铂的磁性生物活性玻璃（MBGs）/Cisplatin 被掺杂到壳聚糖（CS）接枝聚（ε-己内酯）（PCL）纳米纤维中，以在不同 pH 值和温度下控制 Cisplatin 的释放。通过在交变磁场下用 Cs-g-PCL/MBGs/Cisplatin 处理细胞，研究了化疗和热疗的协同作用对 MG-63 骨肉瘤细胞的影响。通过 XRD、FTIR、H NMR、SEM 和 EDX 分析对合成的纳米纤维进行了表征。研究了纤维的生物活性和药物负载效率。BGs/Cisplatin 和 MBGs/Cisplatin 负载的 Cs-g-PCL/MBGs 纳米纤维中没有 Cisplatin 的初始突释，与生理条件相比，在 pH 值为 5.5 和温度为 43°C 时 Cisplatin 的释放速度加快。凋亡/坏死效应表明，100μg/mL 的纳米纤维是杀死 MG-63 细胞的最佳浓度。未来的研究可以集中在将纳米纤维作为植入式装置应用于骨肿瘤旁边，以进行体内骨癌治疗。
