Schiele F, Artur Y, Floc'h A Y, Siest G
Centre de Médecine Préventive, Vandoeuvre-lès-Nancy, France.
Clin Chem. 1988 Apr;34(4):685-90.
We studied several factors affecting biological variation in serum acid phosphatases in a population of 1195 apparently healthy subjects four years old or older. We assayed total acid phosphatase activities in the presence of a transphosphorylating agent and using alpha-naphthyl phosphate as substrate. The main factors modifying total and tartrate-resistant acid phosphatases activities in serum are similar to those observed for total and bone alkaline phosphatases activities: age, sex, and hormonal state (puberty or menopause). The tartrate-inhibited acid phosphatase activity is, however, independent of biological variations. Finally, we propose reference limits for total, tartrate-resistant, and tartrate-inhibited acid phosphatases in serum.
我们在1195名4岁及以上的明显健康受试者群体中研究了影响血清酸性磷酸酶生物学变异的几个因素。我们在转磷酸化剂存在的情况下，以α-萘基磷酸为底物测定了总酸性磷酸酶活性。影响血清中总酸性磷酸酶和抗酒石酸酸性磷酸酶活性的主要因素与总碱性磷酸酶和骨碱性磷酸酶活性中观察到的因素相似：年龄、性别和激素状态（青春期或更年期）。然而，酒石酸抑制的酸性磷酸酶活性与生物学变异无关。最后，我们提出了血清中总酸性磷酸酶、抗酒石酸酸性磷酸酶和酒石酸抑制的酸性磷酸酶的参考限值。
