Lam W K, Eastlund D T, Li C Y, Yam L T
Clin Chem. 1978 Jul;24(7):1105-8.
Spectrophotometry of total acid phosphatase activity in children's sera showed an average value of 22.4 +/- 2.9 and 7.4 +/- 0.8 U/liter, for the hydrolysis of p-nitrophenyl phosphate and alpha-naphthyl phosphate, respectively. Analyses of "band 5b", after electrophoresis on acrylamide gel, gave even higher values. The values for children's sera were much higher than those for sera from adults. The multiplicity of acid phosphatases in sera of children and adults was studied by electrophoresis on acrylamide gel and by chromatography on CM-Sepharose. Both methods showed the major acid phosphatase in children's sera to be an acid pyrophosphatase, band 5b. Its catalytic properties are indistinguishable from the enzyme previously isolated from the spleen of leukemic reticuloendotheliosis.
儿童血清中总酸性磷酸酶活性的分光光度法测定显示，对于对硝基苯磷酸酯和α-萘基磷酸酯的水解，平均值分别为22.4±2.9和7.4±0.8 U/升。在丙烯酰胺凝胶上进行电泳后对“5b带”的分析给出了更高的值。儿童血清的值远高于成人血清的值。通过丙烯酰胺凝胶电泳和CM-琼脂糖凝胶色谱法研究了儿童和成人血清中酸性磷酸酶的多样性。两种方法均显示儿童血清中的主要酸性磷酸酶是酸性焦磷酸酶，即5b带。其催化特性与先前从白血病性网状内皮增生症脾脏中分离出的酶无法区分。
