唾液混样策略用于大规模检测 SARS-CoV-2，通过对无症状受试者的工作组检测，为不同工作场所的潜在应用提供支持。
Saliva Pooling Strategy for the Large-Scale Detection of SARS-CoV-2, Through Working-Groups Testing of Asymptomatic Subjects for Potential Applications in Different Workplaces.
机构信息
Universidad Autónoma de Nayarit, Laboratorio Nacional de Investigación para la Inocuidad Alimentaria (LANIIA)-Unidad Nayarit, Calle Tres S/N, Colonia. Cd. Industrial, Z.P. 63000, Tepic, México.
出版信息
J Occup Environ Med. 2021 Jul 1;63(7):541-547. doi: 10.1097/JOM.0000000000002176.
OBJECTIVE
To perform an improved large-scale SARS-CoV-2 detection on pooled tests of asymptomatic workers.
METHODS
qRT-PCR validation of the SARS-CoV-2 detection in salivae samples and saliva pools and working-group saliva pooling and testing for SARS-CoV-2.
RESULTS
We found a high Cycle threshold correlation (r = 0.9099) between swabs and saliva samples. Then, through the pooling strategy, we detected that 18/360 (5%) of individual saliva samples were SARS-CoV-2 positive. Saliva-pooling efficiency (360 of test sample/30 individual PCR) was higher (5.45) than the reported for swabbing group-testing and we spared 82% of the PCR reagents as well as sampling and personal protection equipment.
CONCLUSION
Through this simplified and less expensive procedure, we detected in a short time asymptomatic-infected SARS-CoV-2-carriers that were isolated from their co-workers, thus, this methodology can be implemented in different workplaces to ensure consumers that employees are not infectious.
目的
对无症状工作者的混合检测样本进行改进的大规模 SARS-CoV-2 检测。
方法
对唾液样本和唾液混合样本中的 SARS-CoV-2 进行 qRT-PCR 验证，并对工作组的唾液混合和 SARS-CoV-2 检测进行研究。
结果
我们发现拭子和唾液样本之间存在高度的循环阈值相关性（r=0.9099）。然后，通过混合策略，我们发现 18/360（5%）份个体唾液样本呈 SARS-CoV-2 阳性。唾液混合效率（360 个检测样本/30 个个体 PCR）高于拭子组检测（5.45），我们节省了 82%的 PCR 试剂以及采样和个人防护设备。
结论
通过这种简化且成本较低的程序，我们在短时间内检测到了与同事隔离的无症状感染 SARS-CoV-2 的携带者，因此，该方法可以在不同的工作场所实施，以确保消费者的员工没有传染性。
Zotero 插件