Department of Urology, Minimally Invasive Surgery Center, The First Affiliated Hospital of Guangzhou Medical University and Guangdong Key Laboratory of Urology, Guangzhou, China.
Department of Urology, Affiliated Dongguan People's Hospital, Southern Medical University, Dongguan, China.
Genet Test Mol Biomarkers. 2021 Feb;25(2):131-139. doi: 10.1089/gtmb.2020.0226.
() expression is acknowledged as a poor clinical prognostic factor in various tumors. However, the clinical characteristics and biological functions of in prostate cancer (PCa) are still to be clarified. The aim of our study was to evaluate the association of expression during PCa progression and its potential role in prognosis. We analyzed mRNA expression of the gene with various clinicopathological features using the Cancer Genome Atlas and GSE21032 dataset. Immunohistochemical assays were used to detect the protein expression levels of in human PCa tissue microarrays. Furthermore, we characterized the role of in PCa progression through experiments using a knockout. Immunohistochemistry and public datasets revealed that expression was increased in PCa with: a high Gleason score; advanced pathological stage; and positive surgical margins. In addition, upregulation of was correlated with shorter biochemical recurrence (BCR)-free survival and overall survival. After we knocked-out in DU145 and LNCaP cells, the phenotypic results showed that the ability of the knockouts to proliferate, migrate, and invade was attenuated; but that apoptosis was promoted. Our data support an oncogenic role for in PCa progression. Moreover, increased expression was identified as an independent factor in predicting bCR-free survival and disease-free survival in PCa patients.
() 表达被认为是各种肿瘤中不良的临床预后因素。然而， 在前列腺癌（PCa）中的临床特征和生物学功能仍有待阐明。本研究旨在评估 在 PCa 进展过程中的表达与预后之间的关联及其潜在作用。
我们使用癌症基因组图谱和 GSE21032 数据集分析了 基因的 mRNA 表达与各种临床病理特征的关系。免疫组织化学检测用于检测人前列腺癌组织微阵列中 蛋白的表达水平。此外，我们通过 基因敲除实验来研究 的作用。免疫组化和公共数据集显示， 在具有高 Gleason 评分、晚期病理分期和阳性手术切缘的 PCa 中表达增加。此外， 上调与生化复发（BCR）无复发生存和总生存时间缩短相关。在 DU145 和 LNCaP 细胞中敲除 后， 表型结果表明，敲除细胞的增殖、迁移和侵袭能力减弱，而凋亡被促进。
我们的数据支持 在 PCa 进展中的致癌作用。此外， 在预测 PCa 患者的 BCR 无复发生存和无病生存方面被确定为独立因素。
