IgA肾病患者中Fc受体样分子的表达谱
Expression profile of Fc receptor-like molecules in patients with IgA nephropathy.
作者信息
Zhong Zhong, Shi Dianchun, Xiao Mengjiao, Fu Dongying, Feng Shaozhen, Kong Qingyu, Li Jianbo, Li Zhijian
机构信息
Department of Nephrology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510080, China; Key Laboratory of Nephrology, National Health Commission and Guangdong Province, Guangzhou, Guangdong 510080, China.
Department of Nephrology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510080, China; Key Laboratory of Nephrology, National Health Commission and Guangdong Province, Guangzhou, Guangdong 510080, China; Division of Nephrology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, School of Medicine, South China University of Technology, Guangzhou, Guangdong 510080, China.
出版信息
Hum Immunol. 2021 Mar;82(3):186-192. doi: 10.1016/j.humimm.2021.01.011. Epub 2021 Feb 14.
BACKGROUND
Fc receptor-like (FCRL) molecules were considered to play a role in the pathogenesis of certain autoimmune diseases. Nonetheless, the clinical significance of FCRLs in IgA nephropathy (IgAN) remains unclear.
OBJECTIVE
This study is aimed at investigating the expression levels of FCRLs molecules in IgAN patients and determining its relevance to disease activity.
METHODS
The mRNA expression levels of FCRLs were determined in peripheral blood mononuclear cells (PBMCs) of 42 IgAN patients and 48 healthy controls by quantitative real-time PCR (qRT-PCR). FCRLs proteins expression in B cells of 25 IgAN patients, 14 patients with non-IgAN glomerulonephritis, and 29 healthy controls were detected by Flow cytometry. The Spearman correlation test was used to assess the correlation of FCRLs expression with clinical parameters of IgAN patients.
RESULTS
Our results indicated significant down-regulation of FCRL2 and FCRL3 mRNA levels in IgAN patients compared to healthy subjects. Surface protein expression of FCRLs molecules confirmed the qRT-PCR results. But FCRL2 and FCRL3 protein levels did not correlate with clinicopathologic phenotypes of IgAN patients. However, we found a significant positively correlation of FCRL2 and FCRL3 mRNA expression with the core 1 β1,3-galactosyltransferase (C1GALT1) and its molecular chaperone (Cosmc) mRNA levels in IgAN patients.
CONCLUSIONS
FCRL2 and FCRL3 expression levels in IgAN patients are significantly decreased and correlated with CIGALT1 and Cosmc mRNA expression.
背景
Fc受体样（FCRL）分子被认为在某些自身免疫性疾病的发病机制中起作用。然而，FCRLs在IgA肾病（IgAN）中的临床意义仍不清楚。
目的
本研究旨在调查IgAN患者中FCRLs分子的表达水平，并确定其与疾病活动的相关性。
方法
通过定量实时PCR（qRT-PCR）测定42例IgAN患者和48例健康对照者外周血单个核细胞（PBMC）中FCRLs的mRNA表达水平。采用流式细胞术检测25例IgAN患者、14例非IgAN肾小球肾炎患者和29例健康对照者B细胞中FCRLs蛋白的表达。采用Spearman相关性检验评估FCRLs表达与IgAN患者临床参数的相关性。
结果
我们的结果表明，与健康受试者相比，IgAN患者中FCRL2和FCRL3 mRNA水平显著下调。FCRLs分子的表面蛋白表达证实了qRT-PCR结果。但FCRL2和FCRL3蛋白水平与IgAN患者的临床病理表型无关。然而，我们发现IgAN患者中FCRL2和FCRL3 mRNA表达与核心1β1,3-半乳糖基转移酶（C1GALT1）及其分子伴侣（Cosmc）mRNA水平呈显著正相关。
结论
IgAN患者中FCRL2和FCRL3表达水平显著降低，且与CIGALT1和Cosmc mRNA表达相关。
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