Lee C Y, Ooi P T, Zunita Z, Noordin M M
Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia 43400, UPM Serdang, Selangor, Malaysia.
Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia 43400, UPM Serdang, Selangor, Malaysia.
Trop Biomed. 2019 Jun 1;36(2):482-487.
Although the economic importance of Haemophilus parasuis infection causing Glasser's disease is prevalent throughout pig farms in Peninsular Malaysia, there is a dearth of knowledge on its actual nature. In this study, a multiplex PCR was performed to screen for three major predominant virulent strains of H. parasuis, which are serotypes 4, 5 or 12 and 13. A total of 175 tissues or bodily fluid samples of various parts were collected from diseased animals from October, 2016 to February, 2018; with total of 62.9% positive detection of H. parasuis. The highest detection was found to be in the pericardial sac fibrin (90.9%) followed by pleural fibrin, lung, pleural fluid, tonsil, pericardial sac, peritoneal fluid, abdominal fibrin, joint fluid, brain and pericardium. Serotype 13 was the highest (40/110) followed by serotype 4(37/110), serotype 5(31/110) and 12 samples were nontypable (12/110). The presence of untypable serotype also drives to further identification of other serotypes in Malaysia.
尽管副猪嗜血杆菌感染引发格拉泽氏病在马来西亚半岛的养猪场中具有普遍的经济重要性，但对其实际性质的了解却十分匮乏。在本研究中，进行了多重聚合酶链反应（multiplex PCR）以筛选副猪嗜血杆菌的三种主要优势致病菌株，即血清型4、5或12以及13。2016年10月至2018年2月期间，从患病动物身上总共采集了175份不同部位的组织或体液样本；副猪嗜血杆菌的阳性检测率总计为62.9%。检测率最高的是在心包膜纤维蛋白中（90.9%），其次是胸膜纤维蛋白、肺、胸水、扁桃体、心包、腹水、腹部纤维蛋白、关节液、脑和心包。血清型13的数量最多（40/110），其次是血清型4（37/110）、血清型5（31/110），有12个样本无法分型（12/110）。无法分型血清型的存在也促使人们进一步鉴定马来西亚的其他血清型。
