索科特拉补血草和钝叶豆瓣绿提取物的植物化学筛选、细胞毒性及抗菌活性

Phytochemical screening, cytotoxic and antimicrobial activities of Limonium socotranum and Peperomia blanda extracts.

作者信息

Al-Madhagi W M, Hashim N M, Ali N A A, Othman R

机构信息

Department of Pharmacy, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.

Center of Natural Products Research and Drug Discovery (CENAR), University of Malaya, 50603, Kuala Lumpur, Malaysia.

出版信息

Trop Biomed. 2019 Mar 1;36(1):11-21.

PMID:33597422

Abstract

Limonium socotranum and Peperomia blanda are used in ethnomedicine to treat several diseases, such as infection, cancer, and inflammation. P. blanda (family: Piperaceae) is from the genus Peperomia, and mostly found in Madagascar, Yemen, USA to South America, while L. socotranum (family: Plumbaginaceae) from the genus Limonium and this species is found only on Socotra Island, Yemen. These plants have attracted great interest in recent years because of their phytochemical contents. Consequently, the current study is aimed to investigate the phytochemical constituents, the cytotoxic and antimicrobial activities of L. socotranum (leaves and stem) and P. blanda extracts. Successive extraction had been performed which resulted in nine crude extracts. Phytochemical screening of the extracts was then conducted using qualitative chemical analysis. The antimicrobial activity of the plant extracts was determined using the well diffusion method against eleven selected pathogenic microbes and the minimum inhibitory concentrations (MIC) were measured. The cytotoxic activities of the plant extracts against MCF-7 and HepG2 cell lines were investigated using sulforhodamine B assay. It was noted that methanol leaves extract from L. socotranum exhibited higher antibacterial activity against Micrococcus luteus (MIC 15.6 µg/mL), Staphylococcus aureus (MIC 125 µg/mL) and Pseudomonas aeruginosa (MIC 125 µg/mL), than stem parts, while petroleum ether extract displayed stronger antifungal activity, with MIC of 125 µg/mL. On the other hand, petroleum ether extract of P. blanda was effective against Gram-positive bacteria and exhibited moderate antifungal activity. Petroleum ether extract of P. blanda displayed cytotoxic activity against MCF-7 cells, with an IC of 4.60 ± 0.02 µg/mL, while the methanol extracts showed higher activity against the HepG2 cell line, with an IC of 13.90 ± 0.14 µg/mL. Phytochemical findings confirmed the presence of flavonoids, alkaloids and terpenoids. The promising obtained results suggest the potential use of these plants in cancer and antimicrobial therapies.

摘要

索科特拉补血草和钝叶豆瓣绿在民族医学中用于治疗多种疾病，如感染、癌症和炎症。钝叶豆瓣绿（胡椒科）属于豆瓣绿属，主要分布在马达加斯加、也门、美国至南美洲，而索科特拉补血草（蓝雪科）属于补血草属，仅在也门的索科特拉岛发现。近年来，这些植物因其植物化学成分而备受关注。因此，本研究旨在调查索科特拉补血草（叶和茎）和钝叶豆瓣绿提取物的植物化学成分、细胞毒性和抗菌活性。进行了连续提取，得到了九种粗提物。然后使用定性化学分析对提取物进行植物化学筛选。采用打孔扩散法测定植物提取物对11种选定致病微生物的抗菌活性，并测定最低抑菌浓度（MIC）。使用磺酰罗丹明B测定法研究植物提取物对MCF-7和HepG2细胞系的细胞毒性活性。值得注意的是，索科特拉补血草的甲醇叶提取物对藤黄微球菌（MIC 15.6 µg/mL）、金黄色葡萄球菌（MIC 125 µg/mL）和铜绿假单胞菌（MIC 125 µg/mL）的抗菌活性高于茎部，而石油醚提取物显示出更强的抗真菌活性，MIC为125 µg/mL。另一方面，钝叶豆瓣绿的石油醚提取物对革兰氏阳性菌有效，并表现出中等抗真菌活性。钝叶豆瓣绿的石油醚提取物对MCF-7细胞具有细胞毒性活性，IC为4.60±0.02 µg/mL，而甲醇提取物对HepG2细胞系表现出更高活性，IC为13.90±0.14 µg/mL。植物化学研究结果证实了黄酮类、生物碱和萜类的存在。所获得的有前景的结果表明这些植物在癌症和抗菌治疗中的潜在用途。